A modified ‘NanoSuit®’ preserves wet samples in high vacuum: direct observations on cells and tissues in field-emission scanning electron microscopy

A modified ‘NanoSuit®’ preserves wet samples in high vacuum: direct observations on cells and tissues in field-emission scanning electron microscopy

Although field-emission scanning electron microscopy (FE-SEM) has proven very useful in biomedical research, the high vacuum required (10−3 to 10−7 Pa) precludes direct observations of living cells and tissues at high resolution and often produces unwanted structural changes. We have previously desc...

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Bibliographic Details
Main Authors: Yasuharu Takaku, Hiroshi Suzuki, Hideya Kawasaki, Isao Ohta, Daisuke Ishii, Satoshi Hirakawa, Takami Tsutsui, Haruko Matsumoto, Sayuri Takehara, Chinatsu Nakane, Kana Sakaida, Chiaki Suzuki, Yoshinori Muranaka, Hirotoshi Kikuchi, Hiroyuki Konno, Masatsugu Shimomura, Takahiko Hariyama
Format: Article
Language:English
Published: The Royal Society 2017-01-01
Series:Royal Society Open Science
Subjects:
field-emission scanning electron microscope (fe-sem)
high vacuo
living specimen
surface shield effect
nanosuit®
Online Access:https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.160887

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