| Summary: | <p>Abstract</p> <p>Background</p> <p><it>Pasteurella </it>species, widely known as indigenous orgganisms in the oral and gastrointestinal floras of many wild and domestic animals, are important pathogens in both animals and humans. Human infections due to <it>Pasteurella </it>species are in most cases associated with infected injuries following animal bites. We encountered a rare case of dual infections caused by different two <it>Pasteurella </it>species occurred in a previously healthy 25-year-old female sustaining injury by a dog-bite.</p> <p>Methodology</p> <p>Exudates from the open wound of her dog-bite site, together with the saliva of the dog were submitted for bacteriological examination. Predominantly appearing grayish-white smooth colonies with almost the same colonial properties but slightly different glistening grown on chocolate and sheep blood agar plates were characterized morphologically by Gram's stain, biochemically by automated instrument using Vitek 2 system using GN cards together with commercially available kit system, ID-Test HN-20 rapid panels, and genetically by sequencing the 16S rRNA genes of the organism using a Taq DyeDeoxy Terminator Cycle Sequencing and a model 3100 DNA sequencer instrument.</p> <p>Results</p> <p>The causative isolates from the dog-bite site were finally identified as <it>P</it>. <it>canis </it>and <it>P</it>. <it>dagmatis </it>from the findings of the morphological, cultural, and biochemical properties together with the comparative sequences of the 16S rRNA genes. Both the isolates were highly susceptible to many antibiotics and the patient was successfully treated with the administration of so-called the first generation cephalosporin, cefazolin followed by so-called the third generation cephalosporin, cefcapene pivoxil. The isolate from the dog was subsequently identified as <it>P</it>. <it>canis</it>, the same species as the isolate from the patient.</p> <p>Conclusions</p> <p>To the best of our knowledge, this was the second report of a dual infection with <it>Pasteurella </it>species consisting of <it>P</it>. <it>dagmatis </it>and <it>P. canis </it>resulting from a dog-bite, followed by the first report of dual infections due to <it>P</it>. <it>dagmatis </it>and <it>P. multocida </it>in 1988. Our isolate finally identified as <it>P</it>. <it>dagmatis </it>was misidentified as <it>P</it>. <it>pneumotripica by </it>means of the Vitek 2 system. The species name "<it>P</it>. <it>dagmatis" </it>was not included in the database of the system. It is also important for routine clinical microbiology laboratories to know the limitation of the automated Vitek 2 system for the accurate identification of <it>Pasteurella </it>species especially <it>P</it>. <it>dagmatis</it>. It should be emphasized that there still exists much room for improvement in Vitek 2 system. Significant improvement of Vitek 2 system especially in the identification of <it>Pasteurella </it>species is urgently desired.</p>
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