Combination of Small Molecule Microarray and Confocal Microscopy Techniques for Live Cell Staining Fluorescent Dye Discovery

• Main Authors: Attila Bokros, Ágnes Medgyesi, Ferhan Ayaydin, László G. Puskás, Gabriella Fábián, Liliána Z. Fehér, Pradeep Kumar Reddy Cingaram, Bhavyashree Suresh, Begüm Peksel, Soujanya Kuntam, Eszter Molnár
• Format: Article
• Language: English
• Published: MDPI AG 2013-08-01
• Series: Molecules
• Subjects: fluorescent dye discovery; high-throughput screening; small molecule microarray; confocal laser scanning microscopy
• Online Access: http://www.mdpi.com/1420-3049/18/8/9999

Discovering new fluorochromes is significantly advanced by high-throughput screening (HTS) methods. In the present study a combination of small molecule microarray (SMM) prescreening and confocal laser scanning microscopy (CLSM) was developed in order to discover novel cell staining fluorescent dyes. Compounds with high native fluorescence were selected from a 14,585-member library and further tested on living cells under the microscope. Eleven compartment-specific, cell-permeable (or plasma membrane-targeted) fluorochromes were identified. Their cytotoxicity was tested and found that between 1–10 micromolar range, they were non-toxic even during long-term incubations.