Phylogenetic analysis and sequencing of inclusion body gene of two Iranian isolates of Carnation etched ring virus

• Main Authors: Mahnaz Ashnaei, Behrouz Jafar Pour, Saeid Malek Zade Shafa Roudi
• Format: Article
• Language: fas
• Published: Shahid Bahonar University of Kerman 2014-08-01
• Series: مجله بیوتکنولوژی کشاورزی
• Subjects: phylogenetic analysis; polymerase chain reaction; carnation etched ring virus
• Online Access: https://jab.uk.ac.ir/article_1308_d41d8cd98f00b204e9800998ecf8427e.pdf

<em>Carnation etched ring virus</em> (CERV) is a member of family <em>caulimoviridae</em>, caulimovirus genus. The virus is the second important virus after <em>Carnation mottle virus</em> to infect carnation.     In order to investigate the presence of this virus in greenhouses carnation plants of Khorasan Razavi (Mashhad) and Markazi (Mahallat) provinces samples which have been showed the symptoms were collected. Primary identification of CERV tested by Indirect enzyme-linked immunosorbent assay. Total DNA was extracted from fresh young leaf tissue by CTAB buffer. Polymerase Chain Reaction (PCR) was carried out using specific primers and fragments of 1500 bp were amplified by PCR. Comparisons were made between these isolates and related sequences of other caulimoviruses available in Genbank. The phylogenetic tree of inclusion body (IB) gene of CERV was drown, by MEGA5 software using Neighbor joining method. The results showed that the maximum similarity of Mahallat isolate was 99.4% to Indian isolate (AJ853858). Comparision of nucleotide sequences was shown the homology of two Iranian isolates together (92.2%) and with the other isolates (90-99.4%). Maximum amino acid changes were indicated in Holland isolate. Although there was a significant difference (60.6-92.9%) in amino acid level between the sequences of the IB region of different caulimovirus.