Establishment of an induced pluripotent stem cell line (CSUi003-A) from fibroblasts of a healthy elderly individual

Establishment of an induced pluripotent stem cell line (CSUi003-A) from fibroblasts of a healthy elderly individual

Skin fibroblasts derived from a 71-year-old healthy woman were reprogrammed into induced pluripotent stem cells (iPSCs) by the transduction of retroviruses expressing OCT4, SOX2, KLF4 and c-MYC, respectively. The generated iPSCs maintained a normal karyotype and expressed various pluripotency marker...

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Main Authors: Hongjun Zhao, Lijing Wang, Junjiao Wu, Jie Ren, Qiong Chen, Hui Luo, Xiaoxia Zuo, Yu Tang
Format: Article
Language:English
Published: Elsevier 2021-05-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506121001720
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spelling doaj-5d1d595c670f47ae9973e153ca711b0b2021-05-30T04:41:34ZengElsevierStem Cell Research1873-50612021-05-0153102326Establishment of an induced pluripotent stem cell line (CSUi003-A) from fibroblasts of a healthy elderly individualHongjun Zhao0Lijing Wang1Junjiao Wu2Jie Ren3Qiong Chen4Hui Luo5Xiaoxia Zuo6Yu Tang7Department of Rheumatology and Immunology, Xiangya Hospital, Central South University, Changsha 410008, China; Provincial Clinical Research Center for Rheumatic and Immunologic Diseases, Xiangya Hospital, Central South University, Changsha 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, ChinaDepartment of Geriatrics, Xiangya Hospital, Central South University, Changsha 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, ChinaDepartment of Rheumatology and Immunology, Xiangya Hospital, Central South University, Changsha 410008, China; Provincial Clinical Research Center for Rheumatic and Immunologic Diseases, Xiangya Hospital, Central South University, Changsha 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, ChinaDepartment of Geriatrics, Xiangya Hospital, Central South University, Changsha 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, ChinaDepartment of Geriatrics, Xiangya Hospital, Central South University, Changsha 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, ChinaDepartment of Rheumatology and Immunology, Xiangya Hospital, Central South University, Changsha 410008, China; Provincial Clinical Research Center for Rheumatic and Immunologic Diseases, Xiangya Hospital, Central South University, Changsha 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, ChinaDepartment of Rheumatology and Immunology, Xiangya Hospital, Central South University, Changsha 410008, China; Provincial Clinical Research Center for Rheumatic and Immunologic Diseases, Xiangya Hospital, Central South University, Changsha 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, ChinaDepartment of Geriatrics, Xiangya Hospital, Central South University, Changsha 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, China; Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; Key Laboratory of Hunan Province in Neurodegenerative Disorders, Central South University, Changsha, Hunan 410008, China; Corresponding author at: Department of Geriatrics, Xiangya Hospital, Central South University, Changsha 410008, China.Skin fibroblasts derived from a 71-year-old healthy woman were reprogrammed into induced pluripotent stem cells (iPSCs) by the transduction of retroviruses expressing OCT4, SOX2, KLF4 and c-MYC, respectively. The generated iPSCs maintained a normal karyotype and expressed various pluripotency markers. Moreover, they could be induced to form embryoid bodies in vitro and teratomas in vivo, indicating the full capacity of differentiating into three germ layers. This iPSC line could be differentiated into multiple cell subtypes for cellular modeling and drug discovery.http://www.sciencedirect.com/science/article/pii/S1873506121001720
collection DOAJ
language English
format Article
sources DOAJ
author Hongjun Zhao
Lijing Wang
Junjiao Wu
Jie Ren
Qiong Chen
Hui Luo
Xiaoxia Zuo
Yu Tang
spellingShingle Hongjun Zhao
Lijing Wang
Junjiao Wu
Jie Ren
Qiong Chen
Hui Luo
Xiaoxia Zuo
Yu Tang
Establishment of an induced pluripotent stem cell line (CSUi003-A) from fibroblasts of a healthy elderly individual
Stem Cell Research
author_facet Hongjun Zhao
Lijing Wang
Junjiao Wu
Jie Ren
Qiong Chen
Hui Luo
Xiaoxia Zuo
Yu Tang
author_sort Hongjun Zhao
title Establishment of an induced pluripotent stem cell line (CSUi003-A) from fibroblasts of a healthy elderly individual
title_short Establishment of an induced pluripotent stem cell line (CSUi003-A) from fibroblasts of a healthy elderly individual
title_full Establishment of an induced pluripotent stem cell line (CSUi003-A) from fibroblasts of a healthy elderly individual
title_fullStr Establishment of an induced pluripotent stem cell line (CSUi003-A) from fibroblasts of a healthy elderly individual
title_full_unstemmed Establishment of an induced pluripotent stem cell line (CSUi003-A) from fibroblasts of a healthy elderly individual
title_sort establishment of an induced pluripotent stem cell line (csui003-a) from fibroblasts of a healthy elderly individual
publisher Elsevier
series Stem Cell Research
issn 1873-5061
publishDate 2021-05-01
description Skin fibroblasts derived from a 71-year-old healthy woman were reprogrammed into induced pluripotent stem cells (iPSCs) by the transduction of retroviruses expressing OCT4, SOX2, KLF4 and c-MYC, respectively. The generated iPSCs maintained a normal karyotype and expressed various pluripotency markers. Moreover, they could be induced to form embryoid bodies in vitro and teratomas in vivo, indicating the full capacity of differentiating into three germ layers. This iPSC line could be differentiated into multiple cell subtypes for cellular modeling and drug discovery.
url http://www.sciencedirect.com/science/article/pii/S1873506121001720
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AT lijingwang establishmentofaninducedpluripotentstemcelllinecsui003afromfibroblastsofahealthyelderlyindividual
AT junjiaowu establishmentofaninducedpluripotentstemcelllinecsui003afromfibroblastsofahealthyelderlyindividual
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