Parallel Recordings of Transmembrane hERG Channel Currents Based on Solvent-Free Lipid Bilayer Microarray

The reconstitution of ion-channel proteins in artificially formed bilayer lipid membranes (BLMs) forms a well-defined system for the functional analysis of ion channels and screening of the effects of drugs that act on these proteins. To improve the efficiency of the BLM reconstitution system, we re...

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Main Authors:	Ryusuke Miyata, Daisuke Tadaki, Daichi Yamaura, Shun Araki, Madoka Sato, Maki Komiya, Teng Ma, Hideaki Yamamoto, Michio Niwano, Ayumi Hirano-Iwata
Format:	Article
Language:	English
Published:	MDPI AG 2021-01-01
Series:	Micromachines
Subjects:	bilayer lipid membrane (BLM) ion channel human <i>ether-a-go-go-</i>related gene (hERG) channel microarray
Online Access:	https://www.mdpi.com/2072-666X/12/1/98

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spelling	doaj-5d02c8f9cf894ae59a914824aba5b6d82021-01-20T00:04:43ZengMDPI AGMicromachines2072-666X2021-01-0112989810.3390/mi12010098Parallel Recordings of Transmembrane hERG Channel Currents Based on Solvent-Free Lipid Bilayer MicroarrayRyusuke Miyata0Daisuke Tadaki1Daichi Yamaura2Shun Araki3Madoka Sato4Maki Komiya5Teng Ma6Hideaki Yamamoto7Michio Niwano8Ayumi Hirano-Iwata9Laboratory for Nanoelectronics and Spintronics, Research Institute of Electrical Communication, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, JapanLaboratory for Nanoelectronics and Spintronics, Research Institute of Electrical Communication, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, JapanLaboratory for Nanoelectronics and Spintronics, Research Institute of Electrical Communication, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, JapanLaboratory for Nanoelectronics and Spintronics, Research Institute of Electrical Communication, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, JapanLaboratory for Nanoelectronics and Spintronics, Research Institute of Electrical Communication, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, JapanLaboratory for Nanoelectronics and Spintronics, Research Institute of Electrical Communication, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, JapanAdvanced Institute for Materials Research, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, JapanLaboratory for Nanoelectronics and Spintronics, Research Institute of Electrical Communication, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, JapanKansei Fukushi Research Institute, Tohoku Fukushi University, 6-149-1 Kunimi-ga-oka, Aoba-ku, Sendai 989-3201, JapanLaboratory for Nanoelectronics and Spintronics, Research Institute of Electrical Communication, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, JapanThe reconstitution of ion-channel proteins in artificially formed bilayer lipid membranes (BLMs) forms a well-defined system for the functional analysis of ion channels and screening of the effects of drugs that act on these proteins. To improve the efficiency of the BLM reconstitution system, we report on a microarray of stable solvent-free BLMs formed in microfabricated silicon (Si) chips, where micro-apertures with well-defined nano- and micro-tapered edges were fabricated. Sixteen micro-wells were manufactured in a chamber made of Teflon<sup>®</sup>, and the Si chips were individually embedded in the respective wells as a recording site. Typically, 11 to 16 BLMs were simultaneously formed with an average BLM number of 13.1, which corresponded to a formation probability of 82%. Parallel recordings of ion-channel activities from multiple BLMs were successfully demonstrated using the human <i>ether-a-go-go-</i>related gene (hERG) potassium channel, of which the relation to arrhythmic side effects following drug treatment is well recognized.https://www.mdpi.com/2072-666X/12/1/98bilayer lipid membrane (BLM)ion channelhuman <i>ether-a-go-go-</i>related gene (hERG) channelmicroarray
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Ryusuke Miyata Daisuke Tadaki Daichi Yamaura Shun Araki Madoka Sato Maki Komiya Teng Ma Hideaki Yamamoto Michio Niwano Ayumi Hirano-Iwata
spellingShingle	Ryusuke Miyata Daisuke Tadaki Daichi Yamaura Shun Araki Madoka Sato Maki Komiya Teng Ma Hideaki Yamamoto Michio Niwano Ayumi Hirano-Iwata Parallel Recordings of Transmembrane hERG Channel Currents Based on Solvent-Free Lipid Bilayer Microarray Micromachines bilayer lipid membrane (BLM) ion channel human <i>ether-a-go-go-</i>related gene (hERG) channel microarray
author_facet	Ryusuke Miyata Daisuke Tadaki Daichi Yamaura Shun Araki Madoka Sato Maki Komiya Teng Ma Hideaki Yamamoto Michio Niwano Ayumi Hirano-Iwata
author_sort	Ryusuke Miyata
title	Parallel Recordings of Transmembrane hERG Channel Currents Based on Solvent-Free Lipid Bilayer Microarray
title_short	Parallel Recordings of Transmembrane hERG Channel Currents Based on Solvent-Free Lipid Bilayer Microarray
title_full	Parallel Recordings of Transmembrane hERG Channel Currents Based on Solvent-Free Lipid Bilayer Microarray
title_fullStr	Parallel Recordings of Transmembrane hERG Channel Currents Based on Solvent-Free Lipid Bilayer Microarray
title_full_unstemmed	Parallel Recordings of Transmembrane hERG Channel Currents Based on Solvent-Free Lipid Bilayer Microarray
title_sort	parallel recordings of transmembrane herg channel currents based on solvent-free lipid bilayer microarray
publisher	MDPI AG
series	Micromachines
issn	2072-666X
publishDate	2021-01-01
description	The reconstitution of ion-channel proteins in artificially formed bilayer lipid membranes (BLMs) forms a well-defined system for the functional analysis of ion channels and screening of the effects of drugs that act on these proteins. To improve the efficiency of the BLM reconstitution system, we report on a microarray of stable solvent-free BLMs formed in microfabricated silicon (Si) chips, where micro-apertures with well-defined nano- and micro-tapered edges were fabricated. Sixteen micro-wells were manufactured in a chamber made of Teflon<sup>®</sup>, and the Si chips were individually embedded in the respective wells as a recording site. Typically, 11 to 16 BLMs were simultaneously formed with an average BLM number of 13.1, which corresponded to a formation probability of 82%. Parallel recordings of ion-channel activities from multiple BLMs were successfully demonstrated using the human <i>ether-a-go-go-</i>related gene (hERG) potassium channel, of which the relation to arrhythmic side effects following drug treatment is well recognized.
topic	bilayer lipid membrane (BLM) ion channel human <i>ether-a-go-go-</i>related gene (hERG) channel microarray
url	https://www.mdpi.com/2072-666X/12/1/98
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Parallel Recordings of Transmembrane hERG Channel Currents Based on Solvent-Free Lipid Bilayer Microarray

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