The histology and the proapoptotic control in the ipsilateral and the contralateral testes following unilateral vasectomy
Objective: The aim of this study was to enlighten both the testicular histology and the genetic aspects of the apoptotic process. Thus an experimental study was designed with a model of unilateral vasectomy. Methods: Twenty-two adult male rats were used and 4 main groups were formed. The first (A),...
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doaj-5d01471b01f24949b5e781bafab0f7932020-11-25T02:47:40ZengPAGEPress PublicationsArchivio Italiano di Urologia e Andrologia1124-35622282-41972015-09-0187319820310.4081/aiua.2015.3.1984472The histology and the proapoptotic control in the ipsilateral and the contralateral testes following unilateral vasectomyAydin Ismet Hazar0Basri Cakiroglu1Ertan Sakalli2Mustafa Bahadir Can Balci3Erkan Eyyupoglu4Tuncay Tas5Orhun Sinanoglu6Pinar Tuzlali7Nusret Can Cilesiz8Taksim Training and Research Hospital, Department of Urology IstanbulHisar Intercontinental Hospital, Department of Urology IstanbulZonguldak State Hospital, Department of Urology, ZonguldakTaksim Training and Research Hospital, Department of Urology IstanbulAmasya Training and Research Hospital, Department of Urology, AmasyaTaksim Training and Research Hospital, Department of Urology IstanbulMaltepe University Medical School, Department of Urology, IstanbulTaksim Training and Research Hospital, Department of Pathology, IstanbulTaksim Training and Research Hospital, Department of Urology IstanbulObjective: The aim of this study was to enlighten both the testicular histology and the genetic aspects of the apoptotic process. Thus an experimental study was designed with a model of unilateral vasectomy. Methods: Twenty-two adult male rats were used and 4 main groups were formed. The first (A), the second (B), the third (C), and the fourth group (D) consisted of 4, 4, 4 and 10 rats respectively. Rats in group A had sham operation while rats in other groups (B, C, D) underwent left vasectomy operation including binding of ductus deferens with a 3/0 silk and cutting a minimum of 1 cm part while preserving the vascular structure under 9x magnification. Rats undergoing unilateral vasectomy were sacrificed at the 1st, 2nd and 8th weeks and their testicular structure and proapoptotic gene proteins were compared with that of the control group undergoing sham operation. Results: We found that vasectomy gradually caused destruction and both ipsilateral and contralateral testicles were affected showing initial apoptosis. Conclusion: The procedure causes destruction in the testicular structure by causing bilateral intratubular germ cell necrosis, unilateral obstruction, increase in the tubular pressure and processes that are aggravated by some probable autoimmune reactions.http://www.pagepressjournals.org/index.php/aiua/article/view/5538TestisSpermatogenesisVasectomyApoptosisBCL-2 protein |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Aydin Ismet Hazar Basri Cakiroglu Ertan Sakalli Mustafa Bahadir Can Balci Erkan Eyyupoglu Tuncay Tas Orhun Sinanoglu Pinar Tuzlali Nusret Can Cilesiz |
spellingShingle |
Aydin Ismet Hazar Basri Cakiroglu Ertan Sakalli Mustafa Bahadir Can Balci Erkan Eyyupoglu Tuncay Tas Orhun Sinanoglu Pinar Tuzlali Nusret Can Cilesiz The histology and the proapoptotic control in the ipsilateral and the contralateral testes following unilateral vasectomy Archivio Italiano di Urologia e Andrologia Testis Spermatogenesis Vasectomy Apoptosis BCL-2 protein |
author_facet |
Aydin Ismet Hazar Basri Cakiroglu Ertan Sakalli Mustafa Bahadir Can Balci Erkan Eyyupoglu Tuncay Tas Orhun Sinanoglu Pinar Tuzlali Nusret Can Cilesiz |
author_sort |
Aydin Ismet Hazar |
title |
The histology and the proapoptotic control in the ipsilateral and the contralateral testes following unilateral vasectomy |
title_short |
The histology and the proapoptotic control in the ipsilateral and the contralateral testes following unilateral vasectomy |
title_full |
The histology and the proapoptotic control in the ipsilateral and the contralateral testes following unilateral vasectomy |
title_fullStr |
The histology and the proapoptotic control in the ipsilateral and the contralateral testes following unilateral vasectomy |
title_full_unstemmed |
The histology and the proapoptotic control in the ipsilateral and the contralateral testes following unilateral vasectomy |
title_sort |
histology and the proapoptotic control in the ipsilateral and the contralateral testes following unilateral vasectomy |
publisher |
PAGEPress Publications |
series |
Archivio Italiano di Urologia e Andrologia |
issn |
1124-3562 2282-4197 |
publishDate |
2015-09-01 |
description |
Objective: The aim of this study was to enlighten both the testicular histology and the genetic aspects of the apoptotic process. Thus an experimental study was designed with a model of unilateral vasectomy. Methods: Twenty-two adult male rats were used and 4 main groups were formed. The first (A), the second (B), the third (C), and the fourth group (D) consisted of 4, 4, 4 and 10 rats respectively. Rats in group A had sham operation while rats in other groups (B, C, D) underwent left vasectomy operation including binding of ductus deferens with a 3/0 silk and cutting a minimum of 1 cm part while preserving the vascular structure under 9x magnification. Rats undergoing unilateral vasectomy were sacrificed at the 1st, 2nd and 8th weeks and their testicular structure and proapoptotic gene proteins were compared with that of the control group undergoing sham operation. Results: We found that vasectomy gradually caused destruction and both ipsilateral and contralateral testicles were affected showing initial apoptosis. Conclusion: The procedure causes destruction in the testicular structure by causing bilateral intratubular germ cell necrosis, unilateral obstruction, increase in the tubular pressure and processes that are aggravated by some probable autoimmune reactions. |
topic |
Testis Spermatogenesis Vasectomy Apoptosis BCL-2 protein |
url |
http://www.pagepressjournals.org/index.php/aiua/article/view/5538 |
work_keys_str_mv |
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