Silencing lncRNA CTD-2510F5.4 inhibits the proliferation, invasion and RACGAP1 expression of Huh-7 human hepatocellular carcinoma cells

• Main Authors: Xian-Jian Wu, Jian Pu
• Format: Article
• Language: English
• Published: Editorial Board of Journal of Hainan Medical University 2021-01-01
• Series: Journal of Hainan Medical University
• Subjects: liver cancer; lncrna; racgap1; proliferation; invasion; apoptosis
• Online Access: http://www.hnykdxxb.com/PDF/202101/04.pdf
• ISSN: 1007-1237; 1007-1237

Objective: To study the effect of lncRNA CTD-2510F5.4 silencing on the proliferation and invasion of huh-7 human hepatocellular carcinoma cells and its possible regulatory relationship with RACGAP1. Methods: The TCGA (The Cancer Genome Atlas) database was used to analyze the expression of lncRNA CTD-2510F5.4 in liver cancer and normal tissues and the correlation with the expression of RACGAP1. CTD-2510F5.4 silencing test was performed on Huh-7 human liver cancer cells, and PCR was used to verify its silencing efficiency. CCK8, Transwell, flow cytometry were used to detect cell proliferation, invasion, and apoptosis, RTPCR, and Western blot. Detect the expression level of RACGAP1. Results: In the TCGA database, lncRNA CTD-2510F5.4 was highly expressed in liver cancer tissues, and it was positively correlated with the expression of RACGAP1 (R=0.85). After silencing CTD- 2510F5.4, the proliferation and invasion of huh-7 human liver cancer cells were inhibited, apoptosis increased, and the mRNA and protein expression of RACGAP1 were downregulated. Conclusion: lncRNA CTD-2510F5.4 may be the oncogenic lncRNA of liver cancer, and RACGAP1 may be the target of lncRNA CTD-2510F5.4. Silencing CTD-2510F5.4 can inhibit the proliferation and invasion of huh-7 human liver cancer cells, and promote apoptosis, which may be achieved by inhibiting the expression of RACGAP1.