Optimization and partial characterization of endoglucanase produced by Streptomyces sp. B-PNG23

• Main Authors: Azzeddine Bettache, Abdelaziz Messis, Estelle Copinet, Mouloud Kecha, Nawel Boucherba, Nabila Belhamiche, Francis Duchiron, Said Benallaoua
• Format: Article
• Language: English
• Published: University of Belgrade, University of Novi Sad 2013-01-01
• Series: Archives of Biological Sciences
• Subjects: Streptomyces; endoglucanases; optimization; characterization
• Online Access: http://www.doiserbia.nb.rs/img/doi/0354-4664/2013/0354-46641302549A.pdf

Streptomyces sp. B-PNG23 was selected as a promising cellulolytic strain and tested for its ability to produce cellulases from agroindustrial residues. A pH value of 7 and temperature of 28°C were found to be optimal for maximum enzyme production. The highest endoglucanase activity was obtained in a medium comprised of wheat bran (2 g/l), yeast extract (2 g/l), NaCl (2 g/l), NH4Cl (2.5 g/l), and (0.4 g/l) of MgSO4. The enzyme was active at a broad range of pH (5-8) and temperatures (40-70°C). The optimum pH and temperature were 6 and 50°C, respectively. In the presence of metal ions Mn2+, Cu2+ and NH4 + the activity of the enzyme increased significantly. The enzyme retained 50% of its activity after heating at 50°C for 6 h. This enzyme could be considered as a thermotolerant biocatalyst that could be utilized in biotechnological applications.