NÍVEIS DE ESTRESSE OXIDATIVO E ALTERAÇÕES CELULARES EM TENISTAS JUVENIS DURANTE UM PERÍODO COMPETITIVO

• Main Authors: Joaquim Maria Ferreira Antunes Neto, Caio Cesar Donadon, Bruna Bergo Nader, Daniela Cristina Sandy Turolle, , Elaine Ribeiro
• Format: Article
• Language: Portuguese
• Published: Instituto Brasileiro de Pesquisa e Ensino em Fisiologia do Exercício 2013-12-01
• Series: Revista Brasileira de Prescrição e Fisiologia do Exercício
• Subjects: Free radicals; Creatine kinase; Oxidative stress; Training
• Online Access: http://www.rbpfex.com.br/index.php/rbpfex/issue/view/42/showToc

Exercise induces an increase in oxygen consumption and energy demand. The increase in oxygen consumption leads to increased production of reactive oxygen species (ROS). Depending on your concentration, ROS react with cellular structures such as membranes, components and elements of the mitochondrial DNA, which can lead to death of DNA (apoptosis). The increased athletic performance in recent years is directly related to research that modulate the antioxidant capacity of athletes, reducing the potential oxidative cell. The aim of this study was to investigate the behavior plasma levels of cellular changes through analyses of lipid peroxidation (TBARS) and leakage of creatine kinase (CK) and the modulation of antioxidant enzyme catalase (CAT) and total shulfhydryl groups (TSG) in erythrocytes of young males tennis players in a competitive period. Three moments were analyzed: Analysis 1: (TBARS: 3.27 ± 0.91 nmol/mL; CK: 235 ± 37 U/L; CAT: 0,53 ± 0,08 k/gHb/min; TSG: 555 ± 49 M); Analysis 2 (TBARS: 5.3 ± 1.15 nmol/mL; CK: 476 ± 34 U/L; CAT: 0.56 ± 0.09 k/gHb/min; TSG: 560 ± 56 M); Analysis 3 (TBARS: 4.6 ± 0.54 nmol/mL; CK: 340 ± 17 U/L; CAT: 0.5 ± 0.07 k/gHb/min; TSG: 547 ± 38 M). The values of antioxidants remained elevates compared to subjects healthy non-athletes, demonstrating adaptation to the training; however, there were significant increases in Analysis 2 and Analysis 3 for TBARS and CK in relationship to Analysis 1 (p<0.001; p<0.05, respectively) and high correlationship index (c = 0.952). In conclusion, we suggest that the use of dosages over simplified, as the plasma CK, may indicate the extent of oxidative stress levels in muscle cells, as well as defining levels of security for determining the modulation of training loads.