<i>Verticillium dahliae</i> VdTHI20, Involved in Pyrimidine Biosynthesis, Is Required for DNA Repair Functions and Pathogenicity

<i>Verticillium dahliae</i> VdTHI20, Involved in Pyrimidine Biosynthesis, Is Required for DNA Repair Functions and Pathogenicity

<i>Verticillium dahliae</i> (<i>V. dahliae</i>) infects roots and colonizes the vascular vessels of host plants, significantly reducing the economic yield of cotton and other crops. In this study, the protein VdTHI20, which is involved in the thiamine biosynthesis pathway, wa...

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Main Authors: Tengfei Qin, Wei Hao, Runrun Sun, Yuqing Li, Yuanyuan Wang, Chunyan Wei, Tao Dong, Bingjie Wu, Na Dong, Weipeng Wang, Jialiang Sun, Qiuyue Yang, Yaxin Zhang, Song Yang, Qinglian Wang
Format: Article
Language:English
Published: MDPI AG 2020-02-01
Series:International Journal of Molecular Sciences
Subjects:
<i>verticillium dahliae</i>
thi20
thiamine biosynthesis
pathogenicity
Online Access:https://www.mdpi.com/1422-0067/21/4/1378
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spelling doaj-5c461ded57784b1d8482defb85c7ac8d2020-11-25T01:45:09ZengMDPI AGInternational Journal of Molecular Sciences1422-00672020-02-01214137810.3390/ijms21041378ijms21041378<i>Verticillium dahliae</i> VdTHI20, Involved in Pyrimidine Biosynthesis, Is Required for DNA Repair Functions and PathogenicityTengfei Qin0Wei Hao1Runrun Sun2Yuqing Li3Yuanyuan Wang4Chunyan Wei5Tao Dong6Bingjie Wu7Na Dong8Weipeng Wang9Jialiang Sun10Qiuyue Yang11Yaxin Zhang12Song Yang13Qinglian Wang14Henan Collaborative Innovation Center of Modern Biological Breeding, Henan Institute of Sciences and Technology, Xinxiang 453003, ChinaSchool of Life Sciences, The Chinese University of Hong Kong, Hong Kong 999077, ChinaHenan Collaborative Innovation Center of Modern Biological Breeding, Henan Institute of Sciences and Technology, Xinxiang 453003, ChinaHenan Collaborative Innovation Center of Modern Biological Breeding, Henan Institute of Sciences and Technology, Xinxiang 453003, ChinaHenan Collaborative Innovation Center of Modern Biological Breeding, Henan Institute of Sciences and Technology, Xinxiang 453003, ChinaHenan Collaborative Innovation Center of Modern Biological Breeding, Henan Institute of Sciences and Technology, Xinxiang 453003, ChinaHenan Collaborative Innovation Center of Modern Biological Breeding, Henan Institute of Sciences and Technology, Xinxiang 453003, ChinaCollege of Agriculture, Liaocheng University, Liaocheng 252059, ChinaHenan Collaborative Innovation Center of Modern Biological Breeding, Henan Institute of Sciences and Technology, Xinxiang 453003, ChinaHenan Collaborative Innovation Center of Modern Biological Breeding, Henan Institute of Sciences and Technology, Xinxiang 453003, ChinaHenan Collaborative Innovation Center of Modern Biological Breeding, Henan Institute of Sciences and Technology, Xinxiang 453003, ChinaHenan Collaborative Innovation Center of Modern Biological Breeding, Henan Institute of Sciences and Technology, Xinxiang 453003, ChinaHenan Collaborative Innovation Center of Modern Biological Breeding, Henan Institute of Sciences and Technology, Xinxiang 453003, ChinaHenan Collaborative Innovation Center of Modern Biological Breeding, Henan Institute of Sciences and Technology, Xinxiang 453003, ChinaHenan Collaborative Innovation Center of Modern Biological Breeding, Henan Institute of Sciences and Technology, Xinxiang 453003, China<i>Verticillium dahliae</i> (<i>V. dahliae</i>) infects roots and colonizes the vascular vessels of host plants, significantly reducing the economic yield of cotton and other crops. In this study, the protein VdTHI20, which is involved in the thiamine biosynthesis pathway, was characterized by knocking out the corresponding <i>VdTHI20</i> gene in <i>V. dahliae</i> via <i>Agrobacterium tumefaciens</i>-mediated transformation (ATMT). The deletion of <i>VdTHI20</i> resulted in several phenotypic defects in vegetative growth and conidiation and in impaired virulence in tobacco seedlings. We show that VdTHI20 increases the tolerance of <i>V. dahliae</i> to UV damage. The impaired vegetative growth of <i>&#916;VdTHI20</i> mutant strains was restored by complementation with a functional copy of the <i>VdTHI20</i> gene or by supplementation with additional thiamine. Furthermore, the root infection and colonization of the <i>&#916;VdTHI20</i> mutant strains were suppressed, as indicated by green fluorescent protein (GFP)-labelling under microscope observation. When the RNAi constructs of <i>VdTHI20</i> were used to transform <i>Nicotiana benthamiana</i>, the transgenic lines expressing dsVdTHI20 showed elevated resistance to <i>V. dahliae</i>. Together, these results suggest that VdTHI20 plays a significant role in the pathogenicity of <i>V. dahliae</i>. In addition, the pathogenesis-related gene <i>VdTHI20</i> exhibits potential for controlling <i>V. dahliae</i> in important crops.https://www.mdpi.com/1422-0067/21/4/1378<i>verticillium dahliae</i>thi20thiamine biosynthesispathogenicity
collection DOAJ
language English
format Article
sources DOAJ
author Tengfei Qin
Wei Hao
Runrun Sun
Yuqing Li
Yuanyuan Wang
Chunyan Wei
Tao Dong
Bingjie Wu
Na Dong
Weipeng Wang
Jialiang Sun
Qiuyue Yang
Yaxin Zhang
Song Yang
Qinglian Wang
spellingShingle Tengfei Qin
Wei Hao
Runrun Sun
Yuqing Li
Yuanyuan Wang
Chunyan Wei
Tao Dong
Bingjie Wu
Na Dong
Weipeng Wang
Jialiang Sun
Qiuyue Yang
Yaxin Zhang
Song Yang
Qinglian Wang
<i>Verticillium dahliae</i> VdTHI20, Involved in Pyrimidine Biosynthesis, Is Required for DNA Repair Functions and Pathogenicity
International Journal of Molecular Sciences
<i>verticillium dahliae</i>
thi20
thiamine biosynthesis
pathogenicity
author_facet Tengfei Qin
Wei Hao
Runrun Sun
Yuqing Li
Yuanyuan Wang
Chunyan Wei
Tao Dong
Bingjie Wu
Na Dong
Weipeng Wang
Jialiang Sun
Qiuyue Yang
Yaxin Zhang
Song Yang
Qinglian Wang
author_sort Tengfei Qin
title <i>Verticillium dahliae</i> VdTHI20, Involved in Pyrimidine Biosynthesis, Is Required for DNA Repair Functions and Pathogenicity
title_short <i>Verticillium dahliae</i> VdTHI20, Involved in Pyrimidine Biosynthesis, Is Required for DNA Repair Functions and Pathogenicity
title_full <i>Verticillium dahliae</i> VdTHI20, Involved in Pyrimidine Biosynthesis, Is Required for DNA Repair Functions and Pathogenicity
title_fullStr <i>Verticillium dahliae</i> VdTHI20, Involved in Pyrimidine Biosynthesis, Is Required for DNA Repair Functions and Pathogenicity
title_full_unstemmed <i>Verticillium dahliae</i> VdTHI20, Involved in Pyrimidine Biosynthesis, Is Required for DNA Repair Functions and Pathogenicity
title_sort <i>verticillium dahliae</i> vdthi20, involved in pyrimidine biosynthesis, is required for dna repair functions and pathogenicity
publisher MDPI AG
series International Journal of Molecular Sciences
issn 1422-0067
publishDate 2020-02-01
description <i>Verticillium dahliae</i> (<i>V. dahliae</i>) infects roots and colonizes the vascular vessels of host plants, significantly reducing the economic yield of cotton and other crops. In this study, the protein VdTHI20, which is involved in the thiamine biosynthesis pathway, was characterized by knocking out the corresponding <i>VdTHI20</i> gene in <i>V. dahliae</i> via <i>Agrobacterium tumefaciens</i>-mediated transformation (ATMT). The deletion of <i>VdTHI20</i> resulted in several phenotypic defects in vegetative growth and conidiation and in impaired virulence in tobacco seedlings. We show that VdTHI20 increases the tolerance of <i>V. dahliae</i> to UV damage. The impaired vegetative growth of <i>&#916;VdTHI20</i> mutant strains was restored by complementation with a functional copy of the <i>VdTHI20</i> gene or by supplementation with additional thiamine. Furthermore, the root infection and colonization of the <i>&#916;VdTHI20</i> mutant strains were suppressed, as indicated by green fluorescent protein (GFP)-labelling under microscope observation. When the RNAi constructs of <i>VdTHI20</i> were used to transform <i>Nicotiana benthamiana</i>, the transgenic lines expressing dsVdTHI20 showed elevated resistance to <i>V. dahliae</i>. Together, these results suggest that VdTHI20 plays a significant role in the pathogenicity of <i>V. dahliae</i>. In addition, the pathogenesis-related gene <i>VdTHI20</i> exhibits potential for controlling <i>V. dahliae</i> in important crops.
topic <i>verticillium dahliae</i>
thi20
thiamine biosynthesis
pathogenicity
url https://www.mdpi.com/1422-0067/21/4/1378
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