Quantitative field testing Rotylenchulus reniformis DNA from metagenomic samples isolated directly from soil.

Quantitative field testing Rotylenchulus reniformis DNA from metagenomic samples isolated directly from soil.

A quantitative PCR procedure targeting the β-tubulin gene determined the number of Rotylenchulus reniformis Linford & Oliveira 1940 in metagenomic DNA samples isolated from soil. Of note, this outcome was in the presence of other soil-dwelling plant parasitic nematodes including its sister genus...

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Main Authors: Kurt Showmaker, Gary W Lawrence, Shien Lu, Clarissa Balbalian, Vincent P Klink
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3241691?pdf=render
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spelling doaj-5be3111a7c6a4db0b6c157a750fac2122020-11-25T01:25:03ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-01612e2895410.1371/journal.pone.0028954Quantitative field testing Rotylenchulus reniformis DNA from metagenomic samples isolated directly from soil.Kurt ShowmakerGary W LawrenceShien LuClarissa BalbalianVincent P KlinkA quantitative PCR procedure targeting the β-tubulin gene determined the number of Rotylenchulus reniformis Linford & Oliveira 1940 in metagenomic DNA samples isolated from soil. Of note, this outcome was in the presence of other soil-dwelling plant parasitic nematodes including its sister genus Helicotylenchus Steiner, 1945. The methodology provides a framework for molecular diagnostics of nematodes from metagenomic DNA isolated directly from soil.http://europepmc.org/articles/PMC3241691?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Kurt Showmaker
Gary W Lawrence
Shien Lu
Clarissa Balbalian
Vincent P Klink
spellingShingle Kurt Showmaker
Gary W Lawrence
Shien Lu
Clarissa Balbalian
Vincent P Klink
Quantitative field testing Rotylenchulus reniformis DNA from metagenomic samples isolated directly from soil.
PLoS ONE
author_facet Kurt Showmaker
Gary W Lawrence
Shien Lu
Clarissa Balbalian
Vincent P Klink
author_sort Kurt Showmaker
title Quantitative field testing Rotylenchulus reniformis DNA from metagenomic samples isolated directly from soil.
title_short Quantitative field testing Rotylenchulus reniformis DNA from metagenomic samples isolated directly from soil.
title_full Quantitative field testing Rotylenchulus reniformis DNA from metagenomic samples isolated directly from soil.
title_fullStr Quantitative field testing Rotylenchulus reniformis DNA from metagenomic samples isolated directly from soil.
title_full_unstemmed Quantitative field testing Rotylenchulus reniformis DNA from metagenomic samples isolated directly from soil.
title_sort quantitative field testing rotylenchulus reniformis dna from metagenomic samples isolated directly from soil.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2011-01-01
description A quantitative PCR procedure targeting the β-tubulin gene determined the number of Rotylenchulus reniformis Linford & Oliveira 1940 in metagenomic DNA samples isolated from soil. Of note, this outcome was in the presence of other soil-dwelling plant parasitic nematodes including its sister genus Helicotylenchus Steiner, 1945. The methodology provides a framework for molecular diagnostics of nematodes from metagenomic DNA isolated directly from soil.
url http://europepmc.org/articles/PMC3241691?pdf=render
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AT garywlawrence quantitativefieldtestingrotylenchulusreniformisdnafrommetagenomicsamplesisolateddirectlyfromsoil
AT shienlu quantitativefieldtestingrotylenchulusreniformisdnafrommetagenomicsamplesisolateddirectlyfromsoil
AT clarissabalbalian quantitativefieldtestingrotylenchulusreniformisdnafrommetagenomicsamplesisolateddirectlyfromsoil
AT vincentpklink quantitativefieldtestingrotylenchulusreniformisdnafrommetagenomicsamplesisolateddirectlyfromsoil
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