Evaluation of the isolation protocols of primary cells from common carp (Cyprinus carpio L.) skin tissue
In this study, pieces of skin from common carp (<em>Cyprinus carpio</em> L.) fish were collected freshly from juveniles from a privet fish farm in Duhok city, Kurdistan-Iraq. Those pieces of skin were cultured In Vitro by using two methods of explanting; direct explants and enzymatic pre...
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| Format: | Article |
| Language: | Arabic |
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University of Mosul, College of Veterinary Medicine
2019-01-01
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| Series: | Iraqi Journal of Veterinary Sciences |
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| Online Access: | https://vetmedmosul.com/article_153837_95b459245f0f26ac469e9cc45040d335.pdf |
| Summary: | In this study, pieces of skin from common carp (<em>Cyprinus carpio</em> L.) fish were collected freshly from juveniles from a privet fish farm in Duhok city, Kurdistan-Iraq. Those pieces of skin were cultured In Vitro by using two methods of explanting; direct explants and enzymatic pre-digested explants of tissue fragments. The results showed a highest cell yield (5×10<sup><sub>3</sub></sup> cell/ml) on passage 2 of enzymatic pre-digested tissues and higher cell growth rate in compare with cell yield (38×10<sup>2</sup> cell/ml) gained from direct explant tissues. Both procedures showed forming of clumped cells colonies in initial passages (P1 and P2), and the morphological changes were developed in both cells to show a fibroblast like appearance thereafter. Such findings are important and advantageous to suggest a faster and more efficient protocol for tissue culture cells isolation with lower cost and better isolation success as well. |
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| ISSN: | 1607-3894 2071-1255 |