Prevalence of Non-O157:H7 Shiga Toxin-Producing Escherichia coli in Diarrheal Stool Samples from Nebraska

Prevalence of Non-O157:H7 Shiga Toxin-Producing Escherichia coli in Diarrheal Stool Samples from Nebraska

We determined the prevalence of Shiga-toxin producing Escherichia coli (STEC) in diarrheal stool samples from Nebraska by three methods: cefixime-tellurite sorbitol MacConkey (CT-SMAC) culture, enterohemorrhagic E. coli (EHEC) enzyme immunoassay, and stx1,2 polymerase chain reaction (PCR). Fourteen...

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Bibliographic Details
Main Authors: Paul D. Fey, R.S. Wickert, M.E. Rupp, T.J. Safranek, S.H. Hinrichs
Format: Article
Language:English
Published: Centers for Disease Control and Prevention 2000-10-01
Series:Emerging Infectious Diseases
Subjects:
0157:H7
Diarrhea
E. Coli
Escherichia Coli
Nebraska
United States
Online Access:https://wwwnc.cdc.gov/eid/article/6/5/00-0513_article
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spelling doaj-5bb09fdca533488d893a42fdb8964eb22020-11-25T01:35:12ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592000-10-016553053310.3201/eid0605.000513Prevalence of Non-O157:H7 Shiga Toxin-Producing Escherichia coli in Diarrheal Stool Samples from NebraskaPaul D. FeyR.S. WickertM.E. RuppT.J. SafranekS.H. HinrichsWe determined the prevalence of Shiga-toxin producing Escherichia coli (STEC) in diarrheal stool samples from Nebraska by three methods: cefixime-tellurite sorbitol MacConkey (CT-SMAC) culture, enterohemorrhagic E. coli (EHEC) enzyme immunoassay, and stx1,2 polymerase chain reaction (PCR). Fourteen (4.2%) of 335 specimens were positive by at least one method (CT-SMAC culture [6 of 14], EHEC enzyme immunoassay [13 of 14], stx1,2 PCR [14 of 14]). Six contained serogroup 0157, while non-0157 were as prevalent as 0157 serogroups.https://wwwnc.cdc.gov/eid/article/6/5/00-0513_article0157:H7DiarrheaE. ColiEscherichia ColiNebraskaUnited States
collection DOAJ
language English
format Article
sources DOAJ
author Paul D. Fey
R.S. Wickert
M.E. Rupp
T.J. Safranek
S.H. Hinrichs
spellingShingle Paul D. Fey
R.S. Wickert
M.E. Rupp
T.J. Safranek
S.H. Hinrichs
Prevalence of Non-O157:H7 Shiga Toxin-Producing Escherichia coli in Diarrheal Stool Samples from Nebraska
Emerging Infectious Diseases
0157:H7
Diarrhea
E. Coli
Escherichia Coli
Nebraska
United States
author_facet Paul D. Fey
R.S. Wickert
M.E. Rupp
T.J. Safranek
S.H. Hinrichs
author_sort Paul D. Fey
title Prevalence of Non-O157:H7 Shiga Toxin-Producing Escherichia coli in Diarrheal Stool Samples from Nebraska
title_short Prevalence of Non-O157:H7 Shiga Toxin-Producing Escherichia coli in Diarrheal Stool Samples from Nebraska
title_full Prevalence of Non-O157:H7 Shiga Toxin-Producing Escherichia coli in Diarrheal Stool Samples from Nebraska
title_fullStr Prevalence of Non-O157:H7 Shiga Toxin-Producing Escherichia coli in Diarrheal Stool Samples from Nebraska
title_full_unstemmed Prevalence of Non-O157:H7 Shiga Toxin-Producing Escherichia coli in Diarrheal Stool Samples from Nebraska
title_sort prevalence of non-o157:h7 shiga toxin-producing escherichia coli in diarrheal stool samples from nebraska
publisher Centers for Disease Control and Prevention
series Emerging Infectious Diseases
issn 1080-6040
1080-6059
publishDate 2000-10-01
description We determined the prevalence of Shiga-toxin producing Escherichia coli (STEC) in diarrheal stool samples from Nebraska by three methods: cefixime-tellurite sorbitol MacConkey (CT-SMAC) culture, enterohemorrhagic E. coli (EHEC) enzyme immunoassay, and stx1,2 polymerase chain reaction (PCR). Fourteen (4.2%) of 335 specimens were positive by at least one method (CT-SMAC culture [6 of 14], EHEC enzyme immunoassay [13 of 14], stx1,2 PCR [14 of 14]). Six contained serogroup 0157, while non-0157 were as prevalent as 0157 serogroups.
topic 0157:H7
Diarrhea
E. Coli
Escherichia Coli
Nebraska
United States
url https://wwwnc.cdc.gov/eid/article/6/5/00-0513_article
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