Prevalence of Non-O157:H7 Shiga Toxin-Producing Escherichia coli in Diarrheal Stool Samples from Nebraska

Prevalence of Non-O157:H7 Shiga Toxin-Producing Escherichia coli in Diarrheal Stool Samples from Nebraska

We determined the prevalence of Shiga-toxin producing Escherichia coli (STEC) in diarrheal stool samples from Nebraska by three methods: cefixime-tellurite sorbitol MacConkey (CT-SMAC) culture, enterohemorrhagic E. coli (EHEC) enzyme immunoassay, and stx1,2 polymerase chain reaction (PCR). Fourteen...

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Bibliographic Details
Main Authors: Paul D. Fey, R.S. Wickert, M.E. Rupp, T.J. Safranek, S.H. Hinrichs
Format: Article
Language:English
Published: Centers for Disease Control and Prevention 2000-10-01
Series:Emerging Infectious Diseases
Subjects:
0157:H7
Diarrhea
E. Coli
Escherichia Coli
Nebraska
United States
Online Access:https://wwwnc.cdc.gov/eid/article/6/5/00-0513_article
Description
Summary:We determined the prevalence of Shiga-toxin producing Escherichia coli (STEC) in diarrheal stool samples from Nebraska by three methods: cefixime-tellurite sorbitol MacConkey (CT-SMAC) culture, enterohemorrhagic E. coli (EHEC) enzyme immunoassay, and stx1,2 polymerase chain reaction (PCR). Fourteen (4.2%) of 335 specimens were positive by at least one method (CT-SMAC culture [6 of 14], EHEC enzyme immunoassay [13 of 14], stx1,2 PCR [14 of 14]). Six contained serogroup 0157, while non-0157 were as prevalent as 0157 serogroups.
ISSN:1080-6040
1080-6059

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