The <i>WUSCHELa</i> (<i>PtoWUSa</i>) is Involved in Developmental Plasticity of Adventitious Root in Poplar

The <i>WUSCHELa</i> (<i>PtoWUSa</i>) is Involved in Developmental Plasticity of Adventitious Root in Poplar

<i>WUSCHEL-RELATED HOMEOBOX</i> (<i>WOX</i>) transcription factors play critical roles in cell fate determination during plant development. As the founding member of the <i>WOX</i> family, <i>WUSCHEL</i> (<i>WUS</i>) is characterized for it...

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Main Authors: Jianbo Li, Huixia Jia, Pei Sun, Jin Zhang, Yongxiu Xia, Jianjun Hu, Lijuan Wang, Mengzhu Lu
Format: Article
Language:English
Published: MDPI AG 2020-02-01
Series:Genes
Subjects:
adventitious roots
auxin
poplar
<i>ptowusa</i>
transgenic plant
Online Access:https://www.mdpi.com/2073-4425/11/2/176
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spelling doaj-5b8c2ac3b23940ad9360e57d2259fb632020-11-25T01:12:58ZengMDPI AGGenes2073-44252020-02-0111217610.3390/genes11020176genes11020176The <i>WUSCHELa</i> (<i>PtoWUSa</i>) is Involved in Developmental Plasticity of Adventitious Root in PoplarJianbo Li0Huixia Jia1Pei Sun2Jin Zhang3Yongxiu Xia4Jianjun Hu5Lijuan Wang6Mengzhu Lu7Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 102300, ChinaState Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, ChinaState Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, ChinaBiosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USAExperimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 102300, ChinaState Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, ChinaState Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, ChinaCollege of Forestry and Biotechnology, Zhejiang Agriculture &amp; Forestry University, Hangzhou 311300, China<i>WUSCHEL-RELATED HOMEOBOX</i> (<i>WOX</i>) transcription factors play critical roles in cell fate determination during plant development. As the founding member of the <i>WOX</i> family, <i>WUSCHEL</i> (<i>WUS</i>) is characterized for its role in maintaining stem cell in meristem. In this study, we investigated the function of <i>Populus tomentosa WUSCHELa</i> (<i>PtoWUSa</i>) in adventitious roots (ARs) in poplar. Expression profile analysis showed that <i>PtoWUSa</i> was not only expressed in shoot apical meristem and stem, but also expressed in ARs. Ectopic expression of <i>PtoWUSa</i> in <i>Arabidopsis</i> resulted in shortened primary root, as well as agravitropism and multiple branches. Overexpression of <i>PtoWUSa</i> in poplar increased the number of ARs but decreased their length. Moreover, the AR tip and lateral root tip became larger and swollen. In addition, the expression of auxin transporter genes <i>PIN-FORMED</i> were downregulated in ARs of transgenic plant. Taken together, these results suggest that <i>PtoWUSa</i> could be involved in AR development in poplar through regulating the polar auxin transport in ARs.https://www.mdpi.com/2073-4425/11/2/176adventitious rootsauxinpoplar<i>ptowusa</i>transgenic plant
collection DOAJ
language English
format Article
sources DOAJ
author Jianbo Li
Huixia Jia
Pei Sun
Jin Zhang
Yongxiu Xia
Jianjun Hu
Lijuan Wang
Mengzhu Lu
spellingShingle Jianbo Li
Huixia Jia
Pei Sun
Jin Zhang
Yongxiu Xia
Jianjun Hu
Lijuan Wang
Mengzhu Lu
The <i>WUSCHELa</i> (<i>PtoWUSa</i>) is Involved in Developmental Plasticity of Adventitious Root in Poplar
Genes
adventitious roots
auxin
poplar
<i>ptowusa</i>
transgenic plant
author_facet Jianbo Li
Huixia Jia
Pei Sun
Jin Zhang
Yongxiu Xia
Jianjun Hu
Lijuan Wang
Mengzhu Lu
author_sort Jianbo Li
title The <i>WUSCHELa</i> (<i>PtoWUSa</i>) is Involved in Developmental Plasticity of Adventitious Root in Poplar
title_short The <i>WUSCHELa</i> (<i>PtoWUSa</i>) is Involved in Developmental Plasticity of Adventitious Root in Poplar
title_full The <i>WUSCHELa</i> (<i>PtoWUSa</i>) is Involved in Developmental Plasticity of Adventitious Root in Poplar
title_fullStr The <i>WUSCHELa</i> (<i>PtoWUSa</i>) is Involved in Developmental Plasticity of Adventitious Root in Poplar
title_full_unstemmed The <i>WUSCHELa</i> (<i>PtoWUSa</i>) is Involved in Developmental Plasticity of Adventitious Root in Poplar
title_sort <i>wuschela</i> (<i>ptowusa</i>) is involved in developmental plasticity of adventitious root in poplar
publisher MDPI AG
series Genes
issn 2073-4425
publishDate 2020-02-01
description <i>WUSCHEL-RELATED HOMEOBOX</i> (<i>WOX</i>) transcription factors play critical roles in cell fate determination during plant development. As the founding member of the <i>WOX</i> family, <i>WUSCHEL</i> (<i>WUS</i>) is characterized for its role in maintaining stem cell in meristem. In this study, we investigated the function of <i>Populus tomentosa WUSCHELa</i> (<i>PtoWUSa</i>) in adventitious roots (ARs) in poplar. Expression profile analysis showed that <i>PtoWUSa</i> was not only expressed in shoot apical meristem and stem, but also expressed in ARs. Ectopic expression of <i>PtoWUSa</i> in <i>Arabidopsis</i> resulted in shortened primary root, as well as agravitropism and multiple branches. Overexpression of <i>PtoWUSa</i> in poplar increased the number of ARs but decreased their length. Moreover, the AR tip and lateral root tip became larger and swollen. In addition, the expression of auxin transporter genes <i>PIN-FORMED</i> were downregulated in ARs of transgenic plant. Taken together, these results suggest that <i>PtoWUSa</i> could be involved in AR development in poplar through regulating the polar auxin transport in ARs.
topic adventitious roots
auxin
poplar
<i>ptowusa</i>
transgenic plant
url https://www.mdpi.com/2073-4425/11/2/176
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