Identification of different oyster species using RAPD-PCR

• Main Authors: Emel Özcan Gökçek, Aynur Lök, Bilge Karahan, Evrim Kurtay
• Format: Article
• Language: English
• Published: Ege University 2017-02-01
• Series: Su Ürünleri Dergisi
• Subjects: genetic analysis; rapd; marker; oyster; alien species; genetik analiz; belirteç; istiridye; istilacı tür
• Online Access: https://dergipark.org.tr/tr/pub/egejfas/issue/27112/292904

Rapid determination of the genetic differences between some oyster samples observed in the Aegean Sea that were assumed as an invasive species and domestic oyster (Ostrea edulis, Linnaeus 1758) using RAPD (Random Amplified Polymorphic DNA)- PCR (Polymerase Chain Reaction) markers was aimed in this study. In this regard, domestic species samples and the other samples collected from Altınoluk coasts which were morphologically different than Ostrea species and were showing similarities to Crassostrea genus were genetically indetified by using 8 RAPD profiles. Total 343 bands were observed in the study. Polymorphism rate was found higher in the samples that might belong to Crassostrea genus. 6 of all loci considered in this study were found highly identical for these species. Total of 16 species-spesific diagnostic bands: 11 bands for the invasive species and 5 bands for the domestic species, were determined.