Preparative isotachophoresis of human plasma high density lipoproteins HDL2 and HDL3.

Preparative isotachophoresis of human plasma high density lipoproteins HDL2 and HDL3.

HDL2 and HDL3 subclasses of human serum HDL were isolated by preparative ultracentrifugation and further analyzed by isotachophoraesis on polyacrylamide gel. The HDL2 divided into six subfractions and the HDL3 into ten subfractions differing in chemical composition and in apolipoproteinl content. Th...

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Bibliographic Details
Main Authors: G Bittilo Bon, G Cazzolato, P Avogaro
Format: Article
Language:English
Published: Elsevier 1981-08-01
Series:Journal of Lipid Research
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520373375
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spelling doaj-5b38a471b9584d37a9649214eae509e82021-04-24T05:50:04ZengElsevierJournal of Lipid Research0022-22751981-08-012269981002Preparative isotachophoresis of human plasma high density lipoproteins HDL2 and HDL3.G Bittilo BonG CazzolatoP AvogaroHDL2 and HDL3 subclasses of human serum HDL were isolated by preparative ultracentrifugation and further analyzed by isotachophoraesis on polyacrylamide gel. The HDL2 divided into six subfractions and the HDL3 into ten subfractions differing in chemical composition and in apolipoproteinl content. The apoA-I/apoA-II ratios differed widely among the various subfractions. The subfractions with the highest apoA-I/apoA-II ratio appeared to have the greatest affinity for cholesterol. The preparative isotachophoresis, used for the first time in this type of investigation, has high resolving power and is reproducible and thus suitable for use in the study of the structure and metabolism of the lipoproteins.http://www.sciencedirect.com/science/article/pii/S0022227520373375
collection DOAJ
language English
format Article
sources DOAJ
author G Bittilo Bon
G Cazzolato
P Avogaro
spellingShingle G Bittilo Bon
G Cazzolato
P Avogaro
Preparative isotachophoresis of human plasma high density lipoproteins HDL2 and HDL3.
Journal of Lipid Research
author_facet G Bittilo Bon
G Cazzolato
P Avogaro
author_sort G Bittilo Bon
title Preparative isotachophoresis of human plasma high density lipoproteins HDL2 and HDL3.
title_short Preparative isotachophoresis of human plasma high density lipoproteins HDL2 and HDL3.
title_full Preparative isotachophoresis of human plasma high density lipoproteins HDL2 and HDL3.
title_fullStr Preparative isotachophoresis of human plasma high density lipoproteins HDL2 and HDL3.
title_full_unstemmed Preparative isotachophoresis of human plasma high density lipoproteins HDL2 and HDL3.
title_sort preparative isotachophoresis of human plasma high density lipoproteins hdl2 and hdl3.
publisher Elsevier
series Journal of Lipid Research
issn 0022-2275
publishDate 1981-08-01
description HDL2 and HDL3 subclasses of human serum HDL were isolated by preparative ultracentrifugation and further analyzed by isotachophoraesis on polyacrylamide gel. The HDL2 divided into six subfractions and the HDL3 into ten subfractions differing in chemical composition and in apolipoproteinl content. The apoA-I/apoA-II ratios differed widely among the various subfractions. The subfractions with the highest apoA-I/apoA-II ratio appeared to have the greatest affinity for cholesterol. The preparative isotachophoresis, used for the first time in this type of investigation, has high resolving power and is reproducible and thus suitable for use in the study of the structure and metabolism of the lipoproteins.
url http://www.sciencedirect.com/science/article/pii/S0022227520373375
work_keys_str_mv AT gbittilobon preparativeisotachophoresisofhumanplasmahighdensitylipoproteinshdl2andhdl3
AT gcazzolato preparativeisotachophoresisofhumanplasmahighdensitylipoproteinshdl2andhdl3
AT pavogaro preparativeisotachophoresisofhumanplasmahighdensitylipoproteinshdl2andhdl3
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