In-Vitro Characterization of mCerulean3_mRuby3 as a Novel FRET Pair with Favorable Bleed-Through Characteristics

In-Vitro Characterization of mCerulean3_mRuby3 as a Novel FRET Pair with Favorable Bleed-Through Characteristics

In previous studies, we encountered substantial problems using the CFP_YFP Förster resonance energy transfer (FRET) pair to analyze protein proximity in the endoplasmic reticulum of live cells. Bleed-through of the donor emission into the FRET channel and overlap of the FRET emission wavele...

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Bibliographic Details
Main Authors: Kira Erismann-Ebner, Anne Marowsky, Michael Arand
Format: Article
Language:English
Published: MDPI AG 2019-02-01
Series:Biosensors
Subjects:
3D fluorescence spectra
FAMPIR
fluorescent protein
excitation
Online Access:https://www.mdpi.com/2079-6374/9/1/33

Internet

https://www.mdpi.com/2079-6374/9/1/33

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