GENETIC DIVERSITY IN SODIUM AZIDE INDUCED WHEAT MUTANTS STUDIED BY SSR MARKERS
Mutations induced artificially way are one of the tools used to increase genetic variation in populations where genetic variation has been shrinking especially due to various reasons one of which is domestication. In this study, Simple Sequence Repeats (SSRs) markers were used to screen genetic dive...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Trakya University
2018-10-01
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| Series: | Trakya University Journal of Natural Sciences |
| Subjects: | |
| Online Access: | https://dergipark.org.tr/en/pub/trkjnat/issue/39729/424305 |
| Summary: | Mutations
induced artificially way are one of the tools used to increase genetic
variation in populations where genetic variation has been shrinking especially
due to various reasons one of which is domestication. In this study, Simple
Sequence Repeats (SSRs) markers were used to screen genetic diversity in sodium
azide (NaN3) induced fourteen fourth-generation advanced wheat
mutant lines. The mean values of polymorphism rate (29.44%), polymorphic
information content (PIC; 0.82), marker index (MI; 1.95) and resolving power
(Rp; 1.31) were calculated according to SSR marker profiles. Two SSRs, Xwmc170
and Xcfd6, were detected as the most polymorphic markers, Xgwm626 proved the highest PIC and MI values,
and Xcfd6 gave the highest Rp value. Unweighted
Pair Group Method with Arithmetic Mean (UPGMA) dendrogram classified 15 plants
into four groups. The Principle Component Analysis (PCA) showed 88.9% of the
total genetic variation. The results obtained in the present study might be
useful for determining the efficiency of NaN3 for creating mutant
wheat lines with enough genetic variability to implement wheat-breeding
programs as germplasm resources. |
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| ISSN: | 2147-0294 2528-9691 |