Pausing controls branching between productive and non-productive pathways during initial transcription in bacteria

RNA synthesis by bacterial RNA polymerase is interrupted by pauses but their role in RNA synthesis is poorly understood. Here the authors use single-molecule FRET and biochemical analysis to show that pausing regulates branching between the abortive and productive outcomes of initial transcription.

Bibliographic Details
Main Authors:	David Dulin, David L. V. Bauer, Anssi M. Malinen, Jacob J. W. Bakermans, Martin Kaller, Zakia Morichaud, Ivan Petushkov, Martin Depken, Konstantin Brodolin, Andrey Kulbachinskiy, Achillefs N. Kapanidis
Format:	Article
Language:	English
Published:	Nature Publishing Group 2018-04-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-018-03902-9

id	doaj-5a7bdeadb61d4f6d961109f5e5564849
record_format	Article
spelling	doaj-5a7bdeadb61d4f6d961109f5e55648492021-05-11T10:20:57ZengNature Publishing GroupNature Communications2041-17232018-04-019111210.1038/s41467-018-03902-9Pausing controls branching between productive and non-productive pathways during initial transcription in bacteriaDavid Dulin0David L. V. Bauer1Anssi M. Malinen2Jacob J. W. Bakermans3Martin Kaller4Zakia Morichaud5Ivan Petushkov6Martin Depken7Konstantin Brodolin8Andrey Kulbachinskiy9Achillefs N. Kapanidis10Biological Physics Research Group, Clarendon Laboratory, Department of Physics, University of OxfordBiological Physics Research Group, Clarendon Laboratory, Department of Physics, University of OxfordBiological Physics Research Group, Clarendon Laboratory, Department of Physics, University of OxfordBiological Physics Research Group, Clarendon Laboratory, Department of Physics, University of OxfordBiological Physics Research Group, Clarendon Laboratory, Department of Physics, University of OxfordInstitut de Recherche en Infectiologie de Montpellier (IRIM) UMR9004 CNRS-Université de MontpellierInstitute of Molecular Genetics, Russian Academy of SciencesDepartment of Bionanoscience, Kavli Institute of Nanoscience, Delft University of TechnologyInstitut de Recherche en Infectiologie de Montpellier (IRIM) UMR9004 CNRS-Université de MontpellierInstitute of Molecular Genetics, Russian Academy of SciencesBiological Physics Research Group, Clarendon Laboratory, Department of Physics, University of OxfordRNA synthesis by bacterial RNA polymerase is interrupted by pauses but their role in RNA synthesis is poorly understood. Here the authors use single-molecule FRET and biochemical analysis to show that pausing regulates branching between the abortive and productive outcomes of initial transcription.https://doi.org/10.1038/s41467-018-03902-9
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	David Dulin David L. V. Bauer Anssi M. Malinen Jacob J. W. Bakermans Martin Kaller Zakia Morichaud Ivan Petushkov Martin Depken Konstantin Brodolin Andrey Kulbachinskiy Achillefs N. Kapanidis
spellingShingle	David Dulin David L. V. Bauer Anssi M. Malinen Jacob J. W. Bakermans Martin Kaller Zakia Morichaud Ivan Petushkov Martin Depken Konstantin Brodolin Andrey Kulbachinskiy Achillefs N. Kapanidis Pausing controls branching between productive and non-productive pathways during initial transcription in bacteria Nature Communications
author_facet	David Dulin David L. V. Bauer Anssi M. Malinen Jacob J. W. Bakermans Martin Kaller Zakia Morichaud Ivan Petushkov Martin Depken Konstantin Brodolin Andrey Kulbachinskiy Achillefs N. Kapanidis
author_sort	David Dulin
title	Pausing controls branching between productive and non-productive pathways during initial transcription in bacteria
title_short	Pausing controls branching between productive and non-productive pathways during initial transcription in bacteria
title_full	Pausing controls branching between productive and non-productive pathways during initial transcription in bacteria
title_fullStr	Pausing controls branching between productive and non-productive pathways during initial transcription in bacteria
title_full_unstemmed	Pausing controls branching between productive and non-productive pathways during initial transcription in bacteria
title_sort	pausing controls branching between productive and non-productive pathways during initial transcription in bacteria
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2018-04-01
description	RNA synthesis by bacterial RNA polymerase is interrupted by pauses but their role in RNA synthesis is poorly understood. Here the authors use single-molecule FRET and biochemical analysis to show that pausing regulates branching between the abortive and productive outcomes of initial transcription.
url	https://doi.org/10.1038/s41467-018-03902-9
work_keys_str_mv	AT daviddulin pausingcontrolsbranchingbetweenproductiveandnonproductivepathwaysduringinitialtranscriptioninbacteria AT davidlvbauer pausingcontrolsbranchingbetweenproductiveandnonproductivepathwaysduringinitialtranscriptioninbacteria AT anssimmalinen pausingcontrolsbranchingbetweenproductiveandnonproductivepathwaysduringinitialtranscriptioninbacteria AT jacobjwbakermans pausingcontrolsbranchingbetweenproductiveandnonproductivepathwaysduringinitialtranscriptioninbacteria AT martinkaller pausingcontrolsbranchingbetweenproductiveandnonproductivepathwaysduringinitialtranscriptioninbacteria AT zakiamorichaud pausingcontrolsbranchingbetweenproductiveandnonproductivepathwaysduringinitialtranscriptioninbacteria AT ivanpetushkov pausingcontrolsbranchingbetweenproductiveandnonproductivepathwaysduringinitialtranscriptioninbacteria AT martindepken pausingcontrolsbranchingbetweenproductiveandnonproductivepathwaysduringinitialtranscriptioninbacteria AT konstantinbrodolin pausingcontrolsbranchingbetweenproductiveandnonproductivepathwaysduringinitialtranscriptioninbacteria AT andreykulbachinskiy pausingcontrolsbranchingbetweenproductiveandnonproductivepathwaysduringinitialtranscriptioninbacteria AT achillefsnkapanidis pausingcontrolsbranchingbetweenproductiveandnonproductivepathwaysduringinitialtranscriptioninbacteria
_version_	1721448279292510208

Pausing controls branching between productive and non-productive pathways during initial transcription in bacteria

Similar Items