Effects and Interactions of Medium Components on Laccase from a Marine-Derived Fungus Using Response Surface Methodology

• Main Authors: Chandralata Raghukumar, Donna D’Souza-Ticlo, Sandeep Garg
• Format: Article
• Language: English
• Published: MDPI AG 2009-11-01
• Series: Marine Drugs
• Subjects: marine-derived basidiomycete; laccase; response surface methodology; Central Composite Design and Plackett Burman Design
• Online Access: http://www.mdpi.com/1660-3397/7/4/672/

The effects of various synthetic medium components and their interactions with each other ultimately impact laccase production in fungi. This was studied using a laccasehyper-producing marine-derived basidiomycete, Cerrena unicolor MTCC 5159. Inducible laccases were produced in the idiophase only after addition of an inducer such as CuSO4. Concentration of carbon and nitrogen acted antagonistically with respect to laccase production. A combination of low nitrogen and high carbon concentration favored both biomass and laccase production. The most favorable combination resulted in 917 U L-1 of laccase. After sufficient growth had occurred, addition of a surfactant such as Tween 80 positively impacted biomass and increased the laccase activity to around 1,300 U L-1. Increasing the surface to volume ratio of the culture vessel further increased its activity to almost 2,000 U L-1.