RNA analysis based on a small number of manually isolated fixed cells (RNA-snMIFxC) to profile stem cells from human deciduous tooth-derived dental pulp cells

RNA analysis based on a small number of manually isolated fixed cells (RNA-snMIFxC) to profile stem cells from human deciduous tooth-derived dental pulp cells

Abstract Background Expression of stemness factors, such as octamer-binding transcription factor 3/4 (OCT3/4), sex determining region Y-box 2 (SOX2), and alkaline phosphatase (ALP) in human deciduous tooth-derived dental pulp cells (HDDPCs) can be assessed through fixation and subsequent immuno- or...

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Bibliographic Details
Main Authors: Emi Inada, Issei Saitoh, Naoko Kubota, Yoko Iwase, Yuki Kiyokawa, Hirofumi Noguchi, Youichi Yamasaki, Masahiro Sato
Format: Article
Language:English
Published: BMC 2021-06-01
Series:Biological Procedures Online
Subjects:
Human deciduous tooth-derived dental pulp cells
Stemness factor
Alkaline phosphatase
Octamer-binding transcription factor 3/4
Manual isolation
Mouthpiece-controlled micropipette
Online Access:https://doi.org/10.1186/s12575-021-00149-5

Internet

https://doi.org/10.1186/s12575-021-00149-5

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