Inheritance and identification of molecular markers associated with a novel dwarfing gene in barley

• Main Authors: Sun Genlou, Guan Weiwei, Sun Dongfa, Ren Xifeng, Li Chengdao
• Format: Article
• Language: English
• Published: BMC 2010-10-01
• Series: BMC Genetics
• Online Access: http://www.biomedcentral.com/1471-2156/11/89

<p>Abstract</p> <p>Background</p> <p>Dwarfing genes have widely been used in barley breeding program. More than 30 types of dwarfs or semidwarfs have been reported, but a few has been exploited in barley breeding because pleiotropic effects of dwarfing genes cause some undesired traits. The plant architecture of newly discovered dwarfing germplasm "Huaai 11" consisted of desirable agronomic traits such as shortened stature and early maturity. Genetic factor controlling the plant height in dwarf line Huaai 11 was investigated.</p> <p>Results</p> <p>The Huaai 11 was crossed with tall varieties Monker, Mpyt, Zhenongda 3, Zaoshu 3, Advance, Huadamai 1, Huadamai 6, Hyproly and Ris01508. All the F₁plants displayed tall trait. Both tall and dwarf plants appeared in all the F₂populations with a 3:1 segregation ratio, suggesting that dwarfism of Huaai 11 is controlled by a single recessive gene, <it>btwd1</it>. Allelism test indicated that this dwarfing gene in the Huaai 11 is nonallelic with the gene <it>br</it>, <it>uzu</it>, <it>sdw1 </it>and <it>denso</it>. Using a double haploid population derived from a cross of Huadamai 6 and Huaai 11 and SSR markers the novel dwarfing gene was mapped onto the long arm of chromosome 7H, and closely linked to Bmac031 and Bmac167 with genetic distance of 2.2 cM.</p> <p>Conclusion</p> <p>Huaai 11 is a new source of dwarf for broadening the genetic base of dwarfism. This dwarf source was controlled by a recessive dwarfing gene <it>btwd1</it>, was mapped onto the long arm of chromosome 7H.</p>