Versatile approach for functional analysis of human proteins and efficient stable cell line generation using FLP-mediated recombination system.

Versatile approach for functional analysis of human proteins and efficient stable cell line generation using FLP-mediated recombination system.

Deciphering a function of a given protein requires investigating various biological aspects. Usually, the protein of interest is expressed with a fusion tag that aids or allows subsequent analyses. Additionally, downregulation or inactivation of the studied gene enables functional studies. Developme...

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Bibliographic Details
Main Authors: Roman J Szczesny, Katarzyna Kowalska, Kamila Klosowska-Kosicka, Aleksander Chlebowski, Ewelina P Owczarek, Zbigniew Warkocki, Tomasz M Kulinski, Dorota Adamska, Kamila Affek, Agata Jedroszkowiak, Anna V Kotrys, Rafal Tomecki, Pawel S Krawczyk, Lukasz S Borowski, Andrzej Dziembowski
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2018-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC5874048?pdf=render

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http://europepmc.org/articles/PMC5874048?pdf=render

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