A first insight into the genetic diversity of <it>Mycobacterium tuberculosis </it>in Dar es Salaam, Tanzania, assessed by spoligotyping

• Main Authors: Mfinanga Sayoki GM, Matee Mecky, Eldholm Vegard, Heun Manfred, Dahle Ulf R
• Format: Article
• Language: English
• Published: BMC 2006-09-01
• Series: BMC Microbiology
• Online Access: http://www.biomedcentral.com/1471-2180/6/76

<p>Abstract</p> <p>Background</p> <p>Tanzania has a high tuberculosis incidence, and genotyping studies of <it>Mycobacterium tuberculosis </it>in the country are necessary in order to improve our understanding of the epidemic. Spoligotyping is a potentially powerful genotyping method due to fast generation of genotyping results, high reproducibility and low operation costs. The recently constructed SpolDB4 database and the model-based program 'Spotclust' can be used to assign isolates to families, subfamilies and variants. The results of a study can thus be analyzed in a global context.</p> <p>Results</p> <p>One hundred forty-seven pulmonary isolates from consecutive tuberculosis patients in Dar es Salaam were spoligotyped. SpolDB4 and 'Spotclust' were used to assign isolates to families, subfamilies and variants. The CAS (37%), LAM (22%) and EAI (17%) families were the most abundant. Despite the dominance of these three families, diversity was high due to variation within <it>M. tuberculosis </it>families. Of the obtained spoligopatterns, 64% were previously unrecorded.</p> <p>Conclusion</p> <p>Spoligotyping is useful to gain an overall understanding of the local TB epidemic. This study demonstrates that the extensive TB epidemic in Dar es Salaam, Tanzania is caused by a few successful <it>M. tuberculosis </it>families, dominated by the CAS family. Import of strains was a minor problem.</p>