Relationships among some pines from subgenera Pinus and Strobus revealed by nuclear EST-microsatellites

• Main Authors: Nikolić Biljana, Kovačević Dragan, Mladenović-Drinić Snežana, Nikolić Ana, Mitić Zorica S., Bojović Srđan, Marin Petar D.
• Format: Article
• Language: English
• Published: Serbian Genetics Society 2018-01-01
• Series: Genetika
• Subjects: Bosnian pine; Macedonian pine; Austrian pine; nuclear EST-SSR markers
• Online Access: http://www.doiserbia.nb.rs/img/doi/0534-0012/2018/0534-00121801069N.pdf
• ISSN: 0534-0012; 1820-6069

Genetic relationships among 12 taxa from subgenera Pinus and Strobus were studied through fourteen microsatellite markers, previously developed for Pinus taeda. To our knowledge, this is the first comparative study of pines using nuclear EST-microsatellites (EST-SSRs). The total number of detected alleles in all investigated taxa was 72 (5.14 in average). The numbers of alleles per locus and PIC values for estimated markers ranged from 3 to 7, and from 0.43 to 0.81, respectively. Presented results are in accordance with majority of previous genetic investigations and infrageneric classification of genus Pinus up to the sectional level, while subsectional position of some species has still not dismissed, especially regarding relict ones. According to nuclear EST-SSRs, Pinus heldreichii is in early-diverging position within subsection Pinaster and shows the greatest closeness with P. halepensis, while Pinus peuce doesn't have basal position within subsection Strobus being more close to P. strobus than to P. wallichiana. Furthermore, the closest connections in subsection Pinus were found between two Pinus nigra subspecies (dalmatica and nigra) as well as between P. sylvestris and P. mugo. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. 173029 and Grant no. TR31068]