Physico-chemical characterization of bacterial and hemagglutinins from the serum of the mud crab Scylla serrata

A naturally occurring hemagglutinin (HA), with activity against bacteria and yeast cells were detected in the serum of Scylla serrata using mammalian erythrocytes (RBC), various bacteria and yeast as indicator cells. The serum gave highest HA titer with rabbit RBC, tripsinized yeast and Vibrio fluvi...

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Main Authors:	SS Jayaraj, R Thiagarajan, M Arumugam, S Vincent
Format:	Article
Language:	English
Published:	University of Modena and Reggio Emilia 2010-02-01
Series:	Invertebrate Survival Journal
Subjects:	hemagglutinin bacterial agglutinin yeast agglutinin serum mud crab Scylla serrata
Online Access:	http://www.isj.unimo.it/articoli/ISJ202.pdf

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spelling	doaj-5983e2bc942842b6889e2dc1b63839692020-11-25T03:08:47ZengUniversity of Modena and Reggio EmiliaInvertebrate Survival Journal1824-307X2010-02-01717988Physico-chemical characterization of bacterial and hemagglutinins from the serum of the mud crab Scylla serrataSS JayarajR ThiagarajanM ArumugamS VincentA naturally occurring hemagglutinin (HA), with activity against bacteria and yeast cells were detected in the serum of Scylla serrata using mammalian erythrocytes (RBC), various bacteria and yeast as indicator cells. The serum gave highest HA titer with rabbit RBC, tripsinized yeast and Vibrio fluvialis. An analysis of the physico-chemical properties of the HA showed it to be specifically dependent on the presence of Ca2+ for its activity, stable between pH 7 to 9 and showed thermal stability between 10 to 30 °C. Further studies demonstrated that the HA is precipitable by ammonium sulphate and TCA. HA- inhibition assays performed with carbohydrates revealed that the serum HA was specific for non-reducing terminal glucose with 1-2 glucosidic linkages. Thus this agglutinin appears to be unique among all the known crustacean agglutinins. http://www.isj.unimo.it/articoli/ISJ202.pdfhemagglutininbacterial agglutininyeast agglutininserummud crabScylla serrata
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	SS Jayaraj R Thiagarajan M Arumugam S Vincent
spellingShingle	SS Jayaraj R Thiagarajan M Arumugam S Vincent Physico-chemical characterization of bacterial and hemagglutinins from the serum of the mud crab Scylla serrata Invertebrate Survival Journal hemagglutinin bacterial agglutinin yeast agglutinin serum mud crab Scylla serrata
author_facet	SS Jayaraj R Thiagarajan M Arumugam S Vincent
author_sort	SS Jayaraj
title	Physico-chemical characterization of bacterial and hemagglutinins from the serum of the mud crab Scylla serrata
title_short	Physico-chemical characterization of bacterial and hemagglutinins from the serum of the mud crab Scylla serrata
title_full	Physico-chemical characterization of bacterial and hemagglutinins from the serum of the mud crab Scylla serrata
title_fullStr	Physico-chemical characterization of bacterial and hemagglutinins from the serum of the mud crab Scylla serrata
title_full_unstemmed	Physico-chemical characterization of bacterial and hemagglutinins from the serum of the mud crab Scylla serrata
title_sort	physico-chemical characterization of bacterial and hemagglutinins from the serum of the mud crab scylla serrata
publisher	University of Modena and Reggio Emilia
series	Invertebrate Survival Journal
issn	1824-307X
publishDate	2010-02-01
description	A naturally occurring hemagglutinin (HA), with activity against bacteria and yeast cells were detected in the serum of Scylla serrata using mammalian erythrocytes (RBC), various bacteria and yeast as indicator cells. The serum gave highest HA titer with rabbit RBC, tripsinized yeast and Vibrio fluvialis. An analysis of the physico-chemical properties of the HA showed it to be specifically dependent on the presence of Ca2+ for its activity, stable between pH 7 to 9 and showed thermal stability between 10 to 30 °C. Further studies demonstrated that the HA is precipitable by ammonium sulphate and TCA. HA- inhibition assays performed with carbohydrates revealed that the serum HA was specific for non-reducing terminal glucose with 1-2 glucosidic linkages. Thus this agglutinin appears to be unique among all the known crustacean agglutinins.
topic	hemagglutinin bacterial agglutinin yeast agglutinin serum mud crab Scylla serrata
url	http://www.isj.unimo.it/articoli/ISJ202.pdf
work_keys_str_mv	AT ssjayaraj physicochemicalcharacterizationofbacterialandhemagglutininsfromtheserumofthemudcrabscyllaserrata AT rthiagarajan physicochemicalcharacterizationofbacterialandhemagglutininsfromtheserumofthemudcrabscyllaserrata AT marumugam physicochemicalcharacterizationofbacterialandhemagglutininsfromtheserumofthemudcrabscyllaserrata AT svincent physicochemicalcharacterizationofbacterialandhemagglutininsfromtheserumofthemudcrabscyllaserrata
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Physico-chemical characterization of bacterial and hemagglutinins from the serum of the mud crab Scylla serrata

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