The Enhancing Effect of Fungal Immunomodulatory Protein-Volvariella Volvacea (FIP-vvo) on Maturation and Function of Mouse Dendritic Cells

• Main Authors: Ju-Pi Li, Yi-Pang Lee, Jung-Chein Ma, Betty-Revon Liu, Nien-Tsu Hsieh, Dz-Chi Chen, Ching-Liang Chu, Ren-In You
• Format: Article
• Language: English
• Published: MDPI AG 2021-05-01
• Series: Life
• Subjects: fungal immunomodulatory proteins (FIPs); FIP-vvo; dendritic cell (DC)
• Online Access: https://www.mdpi.com/2075-1729/11/6/471

Volvariella volvacea, also known as straw mushroom, is a common edible mushroom in Chinese cuisine. It contains many nutrients for human health. A fungal immunomodulatory protein (FIP) has been isolated from <i>V. volvacea</i> and named FIP-vvo. Although the regulatory effects of many FIPs on immunity have been identified, the impact of FIP-vvo in modulating dendritic cells (DCs), which play a key role to connect the innate and the adaptive immunity, is not known. In this study, we aim to study the effect of FIP-vvo on the DC maturation and function. We found that FIP-vvo slightly increased the generation of CD11c⁺ bone marrow-derived DC (BMDC). In addition, the surface expression of MHCII was promoted in BMDCs after the treatment of FIP-vvo, suggesting that FIP-vvo induces DC maturation. Furthermore, FIP-vvo enhanced the ability of BMDCs to activate antigen-specific T cell responses in vitro. In the in vivo study, the FIP-vvo treatment facilitated T cell response in lymph nodes. Therefore, for the first time, our data demonstrated that FIP-vvo promoted DC maturation and function and suggested that FIP-vvo could have benefits for human health by enhancing immunity.