16S rRNA terminal restriction fragment length polymorphism for the characterization of the nasopharyngeal microbiota.

16S rRNA terminal restriction fragment length polymorphism for the characterization of the nasopharyngeal microbiota.

A novel non-culture based 16S rRNA Terminal Restriction Fragment Length Polymorphism (T-RFLP) method using the restriction enzymes Tsp509I and Hpy166II was developed for the characterization of the nasopharyngeal microbiota and validated using recently published 454 pyrosequencing data. 16S rRNA gen...

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Bibliographic Details
Main Authors:	Silvio D Brugger, Laurence Frei, Pascal M Frey, Suzanne Aebi, Kathrin Mühlemann, Markus Hilty
Format:	Article
Language:	English
Published:	Public Library of Science (PLoS) 2012-01-01
Series:	PLoS ONE
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23284951/pdf/?tool=EBI

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