Expression analysis of boiling-stable protein (BsCyp) in response to drought, salt and osmotic treatments in drought tolerant and susceptible cultivars of Triticum aestivum

The effect of osmotic, drought and salt stresses on the induction of boiling-stable proteins were studied in C-306 (drought tolerant) and HD-2004 (drought susceptible) cultivars of Triticum aestivum. Western blotting analysis, using polyclonal antibodies raised against a 20 kDa A. thaliana cyclophil...

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Main Authors:	Gurmeen Rakhra, Arun Dev Sharma, Jatinder Singh, Dhiraj Vyas
Format:	Article
Language:	English
Published:	"Vikol publishing" ST Kolesnichenko V.V. 2011-12-01
Series:	Journal of Stress Physiology & Biochemistry
Subjects:	Boiling-stable proteins cyclophilin water stress wheat
Online Access:	http://www.jspb.ru/issues/2011/N4/JSPB_2011_4_187-196.pdf

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spelling	doaj-58ff4cef463741318a62ccaed1b899282020-11-24T23:48:00Zeng"Vikol publishing" ST Kolesnichenko V.V. Journal of Stress Physiology & Biochemistry1997-08382011-12-0174187196Expression analysis of boiling-stable protein (BsCyp) in response to drought, salt and osmotic treatments in drought tolerant and susceptible cultivars of Triticum aestivumGurmeen RakhraArun Dev SharmaJatinder SinghDhiraj VyasThe effect of osmotic, drought and salt stresses on the induction of boiling-stable proteins were studied in C-306 (drought tolerant) and HD-2004 (drought susceptible) cultivars of Triticum aestivum. Western blotting analysis, using polyclonal antibodies raised against a 20 kDa A. thaliana cyclophilin, revealed the induction of a cross-reacting band (40 kDa, BsCyp) in cv. 306. However no substantial expression was observed in drought sensitive cv. HD-2004, indicating its role in water stress adaptation. Thermostability studies of BsCyp in cv. 306 revealed that BsCyp was stable and can be detected even after 20 min of boiling treatment under all the stresses. Based upon these observations the possible role of BsCyp in water stress tolerance is discussed.http://www.jspb.ru/issues/2011/N4/JSPB_2011_4_187-196.pdfBoiling-stable proteinscyclophilinwater stresswheat
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Gurmeen Rakhra Arun Dev Sharma Jatinder Singh Dhiraj Vyas
spellingShingle	Gurmeen Rakhra Arun Dev Sharma Jatinder Singh Dhiraj Vyas Expression analysis of boiling-stable protein (BsCyp) in response to drought, salt and osmotic treatments in drought tolerant and susceptible cultivars of Triticum aestivum Journal of Stress Physiology & Biochemistry Boiling-stable proteins cyclophilin water stress wheat
author_facet	Gurmeen Rakhra Arun Dev Sharma Jatinder Singh Dhiraj Vyas
author_sort	Gurmeen Rakhra
title	Expression analysis of boiling-stable protein (BsCyp) in response to drought, salt and osmotic treatments in drought tolerant and susceptible cultivars of Triticum aestivum
title_short	Expression analysis of boiling-stable protein (BsCyp) in response to drought, salt and osmotic treatments in drought tolerant and susceptible cultivars of Triticum aestivum
title_full	Expression analysis of boiling-stable protein (BsCyp) in response to drought, salt and osmotic treatments in drought tolerant and susceptible cultivars of Triticum aestivum
title_fullStr	Expression analysis of boiling-stable protein (BsCyp) in response to drought, salt and osmotic treatments in drought tolerant and susceptible cultivars of Triticum aestivum
title_full_unstemmed	Expression analysis of boiling-stable protein (BsCyp) in response to drought, salt and osmotic treatments in drought tolerant and susceptible cultivars of Triticum aestivum
title_sort	expression analysis of boiling-stable protein (bscyp) in response to drought, salt and osmotic treatments in drought tolerant and susceptible cultivars of triticum aestivum
publisher	"Vikol publishing" ST Kolesnichenko V.V.
series	Journal of Stress Physiology & Biochemistry
issn	1997-0838
publishDate	2011-12-01
description	The effect of osmotic, drought and salt stresses on the induction of boiling-stable proteins were studied in C-306 (drought tolerant) and HD-2004 (drought susceptible) cultivars of Triticum aestivum. Western blotting analysis, using polyclonal antibodies raised against a 20 kDa A. thaliana cyclophilin, revealed the induction of a cross-reacting band (40 kDa, BsCyp) in cv. 306. However no substantial expression was observed in drought sensitive cv. HD-2004, indicating its role in water stress adaptation. Thermostability studies of BsCyp in cv. 306 revealed that BsCyp was stable and can be detected even after 20 min of boiling treatment under all the stresses. Based upon these observations the possible role of BsCyp in water stress tolerance is discussed.
topic	Boiling-stable proteins cyclophilin water stress wheat
url	http://www.jspb.ru/issues/2011/N4/JSPB_2011_4_187-196.pdf
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Expression analysis of boiling-stable protein (BsCyp) in response to drought, salt and osmotic treatments in drought tolerant and susceptible cultivars of Triticum aestivum

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