A Rapid Method for Detection of Salmonella in Milk Based on Extraction of mRNA Using Magnetic Capture Probes and RT-qPCR

A Rapid Method for Detection of Salmonella in Milk Based on Extraction of mRNA Using Magnetic Capture Probes and RT-qPCR

Magnetic separation is an efficient method for target enrichment and elimination of inhibitors in the molecular detection systems for foodborne pathogens. In this study, we prepared magnetic capture probes by modifying oligonucleotides complementary to target sequences on the surface of amino-modifi...

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Bibliographic Details
Main Authors: Yalong Bai, Yan Cui, Yujuan Suo, Chunlei Shi, Dapeng Wang, Xianming Shi
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-04-01
Series:Frontiers in Microbiology
Subjects:
amino-modified silica-coated magnetic nanoparticles
magnetic capture probes
Salmonella
RT-qPCR
milk
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2019.00770/full

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