Bent DNA Bows as Sensing Amplifiers for Detecting DNA-Interacting Salts and Molecules

• Main Authors: Jack Freeland, Lihua Zhang, Shih-Ting Wang, Mason Ruiz, Yong Wang
• Format: Article
• Language: English
• Published: MDPI AG 2020-05-01
• Series: Sensors
• Subjects: nucleic acids; interactions; elasticity; bending; molecular spring
• Online Access: https://www.mdpi.com/1424-8220/20/11/3112

Due to the central role of DNA, its interactions with inorganic salts and small organic molecules are important. For example, such interactions play important roles in various fundamental cellular processes in living systems and are involved in many DNA-damage related diseases. Strategies to improve the sensitivity of existing techniques for studying DNA interactions with other molecules would be appreciated in situations where the interactions are too weak. Here we report our development and demonstration of bent DNA bows for amplifying, sensing, and detecting the interactions of 14 inorganic salts and small organic molecules with DNA. With the bent DNA bows, these interactions were easily visualized and quantified in gel electrophoresis, which were difficult to measure without bending. In addition, the strength of the interactions of DNA with the various salts/molecules were quantified using the modified Hill equation. This work highlights the amplification effects of the bending elastic energy stored in the DNA bows and the potential use of the DNA bows for quantitatively measuring DNA interactions with small molecules as simple economic methods; it may also pave the way for exploiting the bent DNA bows for other applications such as screening DNA-interacting molecules and drugs.