Mycobacterium tuberculosis DNA Detection Using Surface Plasmon Resonance Modulated by Telecommunication Wavelength
A surface plasmon resonance sensor for Mycobacterium tuberculosis (MTB) deoxyribonucleic acid (DNA) is developed using repeatable telecommunication wavelength modulation based on optical fiber communications laser wavelength and stability. MTB DNA concentrations of 1 μg/mL and 10 μg/mL were successf...
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MDPI AG
2013-12-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/14/1/458 |
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doaj-5887e46e4fb64e14ac7524db1d8845ef2020-11-25T02:09:17ZengMDPI AGSensors1424-82202013-12-0114145846710.3390/s140100458s140100458Mycobacterium tuberculosis DNA Detection Using Surface Plasmon Resonance Modulated by Telecommunication WavelengthShih-Hsiang Hsu0Yan-Yu Lin1Shao-Hsi Lu2I-Fang Tsai3Yen-Ta Lu4Hsin-Tsung Ho5Department of Electronic Engineering, National Taiwan University of Science and Technology, No. 43, Sec. 4, Keelung Rd., Taipei 10607, TaiwanDepartment of Electronic Engineering, National Taiwan University of Science and Technology, No. 43, Sec. 4, Keelung Rd., Taipei 10607, TaiwanDepartment of Electronic Engineering, National Taiwan University of Science and Technology, No. 43, Sec. 4, Keelung Rd., Taipei 10607, TaiwanDepartment of Medical Research, Mackay Memorial Hospital, Taipei 25160, TaiwanDivision of Chest Medicine, Department of Internal Medicine, Mackay Memorial Hospital, Taipei 10449, TaiwanDepartment of Medicine, Mackay Medical College, 25245, New Taipei City, TaiwanA surface plasmon resonance sensor for Mycobacterium tuberculosis (MTB) deoxyribonucleic acid (DNA) is developed using repeatable telecommunication wavelength modulation based on optical fiber communications laser wavelength and stability. MTB DNA concentrations of 1 μg/mL and 10 μg/mL were successfully demonstrated to have the same spectral half-width in the dip for optimum coupling. The sensitivity was shown to be −0.087 dB/(μg/mL) at all applied telecommunication wavelengths and the highest sensitivity achieved was 115 ng/mL without thiolated DNA immobilization onto a gold plate, which is better than the sensor limit of 400 ng/mL possible with commercial biosensor equipment.http://www.mdpi.com/1424-8220/14/1/458Mycobacterium tuberculosissurface plasmon resonancewavelength modulation |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Shih-Hsiang Hsu Yan-Yu Lin Shao-Hsi Lu I-Fang Tsai Yen-Ta Lu Hsin-Tsung Ho |
| spellingShingle |
Shih-Hsiang Hsu Yan-Yu Lin Shao-Hsi Lu I-Fang Tsai Yen-Ta Lu Hsin-Tsung Ho Mycobacterium tuberculosis DNA Detection Using Surface Plasmon Resonance Modulated by Telecommunication Wavelength Sensors Mycobacterium tuberculosis surface plasmon resonance wavelength modulation |
| author_facet |
Shih-Hsiang Hsu Yan-Yu Lin Shao-Hsi Lu I-Fang Tsai Yen-Ta Lu Hsin-Tsung Ho |
| author_sort |
Shih-Hsiang Hsu |
| title |
Mycobacterium tuberculosis DNA Detection Using Surface Plasmon Resonance Modulated by Telecommunication Wavelength |
| title_short |
Mycobacterium tuberculosis DNA Detection Using Surface Plasmon Resonance Modulated by Telecommunication Wavelength |
| title_full |
Mycobacterium tuberculosis DNA Detection Using Surface Plasmon Resonance Modulated by Telecommunication Wavelength |
| title_fullStr |
Mycobacterium tuberculosis DNA Detection Using Surface Plasmon Resonance Modulated by Telecommunication Wavelength |
| title_full_unstemmed |
Mycobacterium tuberculosis DNA Detection Using Surface Plasmon Resonance Modulated by Telecommunication Wavelength |
| title_sort |
mycobacterium tuberculosis dna detection using surface plasmon resonance modulated by telecommunication wavelength |
| publisher |
MDPI AG |
| series |
Sensors |
| issn |
1424-8220 |
| publishDate |
2013-12-01 |
| description |
A surface plasmon resonance sensor for Mycobacterium tuberculosis (MTB) deoxyribonucleic acid (DNA) is developed using repeatable telecommunication wavelength modulation based on optical fiber communications laser wavelength and stability. MTB DNA concentrations of 1 μg/mL and 10 μg/mL were successfully demonstrated to have the same spectral half-width in the dip for optimum coupling. The sensitivity was shown to be −0.087 dB/(μg/mL) at all applied telecommunication wavelengths and the highest sensitivity achieved was 115 ng/mL without thiolated DNA immobilization onto a gold plate, which is better than the sensor limit of 400 ng/mL possible with commercial biosensor equipment. |
| topic |
Mycobacterium tuberculosis surface plasmon resonance wavelength modulation |
| url |
http://www.mdpi.com/1424-8220/14/1/458 |
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