Live and fixed imaging of translation sites at single mRNA resolution in the Drosophila embryo

Live and fixed imaging of translation sites at single mRNA resolution in the Drosophila embryo

Summary: Significant regulation of gene expression is mediated at the translation level. Here, we describe protocols for imaging and analysis of translation at single mRNA resolution in both fixed and living Drosophila embryos. These protocols use the SunTag system, in which the protein of interest...

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Bibliographic Details
Main Authors: Daisy J. Vinter, Caroline Hoppe, Hilary L. Ashe
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
Cell Biology
Developmental biology
Gene Expression
In Situ Hybridization
Microscopy
Model Organisms
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721005189

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