Rapid Separation of All Four Tocopherol Homologues in Selected Fruit Seeds via Supercritical Fluid Chromatography Using a Solid-Core C18 Column

Rapid Separation of All Four Tocopherol Homologues in Selected Fruit Seeds via Supercritical Fluid Chromatography Using a Solid-Core C18 Column

Tocopherol separations employing the same Kinetex™ C18 column via supercritical fluid chromatography (SFC) and reversed-phase liquid chromatography (RP-LC) were compared. The application of the SFC system with UV diode array detection (DAD) resulted in rapid separation of all four tocopherol homolog...

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Main Authors: Elise Urvaka, Inga Mišina, Arianne Soliven, Paweł Górnaś
Format: Article
Language:English
Published: Hindawi Limited 2019-01-01
Series:Journal of Chemistry
Online Access:http://dx.doi.org/10.1155/2019/5307340
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spelling doaj-58508869819e4cf7853d13beb349d85d2020-11-24T21:47:59ZengHindawi LimitedJournal of Chemistry2090-90632090-90712019-01-01201910.1155/2019/53073405307340Rapid Separation of All Four Tocopherol Homologues in Selected Fruit Seeds via Supercritical Fluid Chromatography Using a Solid-Core C18 ColumnElise Urvaka0Inga Mišina1Arianne Soliven2Paweł Górnaś3Institute of Horticulture, Latvia University of Life Sciences and Technologies, Graudu 1, Dobele LV-3701, LatviaInstitute of Horticulture, Latvia University of Life Sciences and Technologies, Graudu 1, Dobele LV-3701, LatviaGrupo de Análisis de Contaminantes Traza, Cátedra de Farmacognosia y Productos Naturales, Facultad de Química, Universidad de la República, General Flores 2124, 11800 Montevideo, UruguayInstitute of Horticulture, Latvia University of Life Sciences and Technologies, Graudu 1, Dobele LV-3701, LatviaTocopherol separations employing the same Kinetex™ C18 column via supercritical fluid chromatography (SFC) and reversed-phase liquid chromatography (RP-LC) were compared. The application of the SFC system with UV diode array detection (DAD) resulted in rapid separation of all four tocopherol homologues with a total analysis time below 2 min. The RP-LC approach could not separate the isomers β and γ. The developed SFC-DAD method was precise, accurate, and most importantly more environmentally friendlier compared to the RP-LC method due to the 125-fold decrease in methanol consumption. The present study illustrated the selectivity differences between LC and SFC and how the C18 column can be used for tocopherol characterization. The optimized SFC method was successfully applied for the tocopherol determination in the seeds of nine different fruit species.http://dx.doi.org/10.1155/2019/5307340
collection DOAJ
language English
format Article
sources DOAJ
author Elise Urvaka
Inga Mišina
Arianne Soliven
Paweł Górnaś
spellingShingle Elise Urvaka
Inga Mišina
Arianne Soliven
Paweł Górnaś
Rapid Separation of All Four Tocopherol Homologues in Selected Fruit Seeds via Supercritical Fluid Chromatography Using a Solid-Core C18 Column
Journal of Chemistry
author_facet Elise Urvaka
Inga Mišina
Arianne Soliven
Paweł Górnaś
author_sort Elise Urvaka
title Rapid Separation of All Four Tocopherol Homologues in Selected Fruit Seeds via Supercritical Fluid Chromatography Using a Solid-Core C18 Column
title_short Rapid Separation of All Four Tocopherol Homologues in Selected Fruit Seeds via Supercritical Fluid Chromatography Using a Solid-Core C18 Column
title_full Rapid Separation of All Four Tocopherol Homologues in Selected Fruit Seeds via Supercritical Fluid Chromatography Using a Solid-Core C18 Column
title_fullStr Rapid Separation of All Four Tocopherol Homologues in Selected Fruit Seeds via Supercritical Fluid Chromatography Using a Solid-Core C18 Column
title_full_unstemmed Rapid Separation of All Four Tocopherol Homologues in Selected Fruit Seeds via Supercritical Fluid Chromatography Using a Solid-Core C18 Column
title_sort rapid separation of all four tocopherol homologues in selected fruit seeds via supercritical fluid chromatography using a solid-core c18 column
publisher Hindawi Limited
series Journal of Chemistry
issn 2090-9063
2090-9071
publishDate 2019-01-01
description Tocopherol separations employing the same Kinetex™ C18 column via supercritical fluid chromatography (SFC) and reversed-phase liquid chromatography (RP-LC) were compared. The application of the SFC system with UV diode array detection (DAD) resulted in rapid separation of all four tocopherol homologues with a total analysis time below 2 min. The RP-LC approach could not separate the isomers β and γ. The developed SFC-DAD method was precise, accurate, and most importantly more environmentally friendlier compared to the RP-LC method due to the 125-fold decrease in methanol consumption. The present study illustrated the selectivity differences between LC and SFC and how the C18 column can be used for tocopherol characterization. The optimized SFC method was successfully applied for the tocopherol determination in the seeds of nine different fruit species.
url http://dx.doi.org/10.1155/2019/5307340
work_keys_str_mv AT eliseurvaka rapidseparationofallfourtocopherolhomologuesinselectedfruitseedsviasupercriticalfluidchromatographyusingasolidcorec18column
AT ingamisina rapidseparationofallfourtocopherolhomologuesinselectedfruitseedsviasupercriticalfluidchromatographyusingasolidcorec18column
AT ariannesoliven rapidseparationofallfourtocopherolhomologuesinselectedfruitseedsviasupercriticalfluidchromatographyusingasolidcorec18column
AT pawełgornas rapidseparationofallfourtocopherolhomologuesinselectedfruitseedsviasupercriticalfluidchromatographyusingasolidcorec18column
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