Quantitative Microdialysis: Experimental Protocol and Software for Small Molecule Protein Affinity Determination and for Exclusion of Compounds with Poor Physicochemical Properties
Quantitative microdialysis is a traditional biophysical affinity determination technique. In the development of the detailed experimental protocol presented, we used commercially available equipment, rapid equilibrium dialysis (RED) devices (ThermoFisher Scientific), which means that it is open to m...
Main Authors: | Steven Shave, Nhan T. Pham, Connor B. Śmieja, Manfred Auer |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2020-07-01
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Series: | Methods and Protocols |
Subjects: | |
Online Access: | https://www.mdpi.com/2409-9279/3/3/55 |
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