Detection molecular of Rangelia vitalii in dogs from Parana State, Southern Brazil

• Main Authors: Bianca Ressetti da Silva, Morgana de Fátima Kuteques Ferreira, Gabriela Maffezzolli, Marília de Oliveira Koch, Olair Carlos Beltrame, Isis Indaiara Gonçalves Granjeiro Taques, Amanda Noéli da Silva Campos, Daniel Moura de Aguiar, Rosangela Locatelli Dittrich
• Format: Article
• Language: English
• Published: Colégio Brasileiro de Parasitologia Veterinaria 2018-12-01
• Series: Revista Brasileira de Parasitologia Veterinária
• Subjects: Cão; hemoparasita; nambiuvú; rangeliose; doença transmitida por carrapato
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018005014102&lng=en&tlng=en

Abstract Rangelia vitalii infects erythrocytes, leukocytes and endothelial cells of dogs. The present study aimed to report the molecular detection confirmed by sequencing of R. vitalii in the state of Paraná, as well as describe the clinical, hematological and biochemical alterations of the infected dogs. Three sick dogs from the metropolitan area of Curitiba, PR, Brazil, underwent a physical exam, and laboratory tests included hematology, biochemistry, polymerase chain reaction (PCR), and gene sequencing. Clinical signs included apathy, anorexia, and hemorrhage. Intra-erythrocytic and extracellular piroplasms were found on peripheral blood smears from all three dogs. Blood samples from these animals were positive for Babesia sp. by PCR targeting 18S rRNA. PCR products from all three dogs were sequenced, and BLAST analysis showed that the PCR-generated sequences were highly homologous with those of R. vitalii previously reported. Hematologic findings included severe anemia, shift of neutrophils to the regenerative left, and thrombocytopenia. Serum urea levels were increased in all three dogs, and direct bilirubin levels were elevated in one dog.