Preparative separation and purification of deoxyschizandrin from Schisandrae Sphenantherae Fructus by high-speed counter-current chromatography

Preparative separation and purification of deoxyschizandrin from Schisandrae Sphenantherae Fructus by high-speed counter-current chromatography

A high-speed counter-current chromatography (HSCCC) method was successfully developed for the preparative separation and purification of deoxyschizandrin from Schisandrae Sphenantherae Fructus in one step. The purity of deoxyschizandrin was 98.5%, and the structure was identified by MS, UV and NMR....

Full description

Bibliographic Details
Main Authors: Xin-Ying Li, Mei Yang, Jie-Yun Huang, Xiao-Xue Yu, Min-Qian Zhao, Zhi-Kun Liang, Zhi-Sheng Xie, Xin-Jun Xu
Format: Article
Language:English
Published: Elsevier 2013-12-01
Series:Journal of Pharmaceutical Analysis
Online Access:http://www.sciencedirect.com/science/article/pii/S2095177913000713
id doaj-58299bc0d9e742549fd52504bf272d13
record_format Article
spelling doaj-58299bc0d9e742549fd52504bf272d132021-04-02T09:53:32ZengElsevierJournal of Pharmaceutical Analysis2095-17792013-12-0136429433Preparative separation and purification of deoxyschizandrin from Schisandrae Sphenantherae Fructus by high-speed counter-current chromatographyXin-Ying Li0Mei Yang1Jie-Yun Huang2Xiao-Xue Yu3Min-Qian Zhao4Zhi-Kun Liang5Zhi-Sheng Xie6Xin-Jun Xu7School of Pharmaceutical Sciences, Sun Yat-Sen University, No. 132, East Waihuan Rd., Guangzhou Higher Education Mega Center, Guangzhou 510006, China; Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, ChinaSchool of Pharmaceutical Sciences, Sun Yat-Sen University, No. 132, East Waihuan Rd., Guangzhou Higher Education Mega Center, Guangzhou 510006, China; Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, ChinaSchool of Pharmaceutical Sciences, Sun Yat-Sen University, No. 132, East Waihuan Rd., Guangzhou Higher Education Mega Center, Guangzhou 510006, China; Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, ChinaSchool of Pharmaceutical Sciences, Sun Yat-Sen University, No. 132, East Waihuan Rd., Guangzhou Higher Education Mega Center, Guangzhou 510006, China; Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, ChinaSchool of Pharmaceutical Sciences, Sun Yat-Sen University, No. 132, East Waihuan Rd., Guangzhou Higher Education Mega Center, Guangzhou 510006, China; Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, ChinaSchool of Pharmaceutical Sciences, Sun Yat-Sen University, No. 132, East Waihuan Rd., Guangzhou Higher Education Mega Center, Guangzhou 510006, China; Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, ChinaSchool of Pharmaceutical Sciences, Sun Yat-Sen University, No. 132, East Waihuan Rd., Guangzhou Higher Education Mega Center, Guangzhou 510006, China; Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, ChinaSchool of Pharmaceutical Sciences, Sun Yat-Sen University, No. 132, East Waihuan Rd., Guangzhou Higher Education Mega Center, Guangzhou 510006, China; Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, China; Corresponding author at: School of Pharmaceutical Sciences, Sun Yat-Sen University, No. 132, East Waihuan Rd., Guangzhou Higher Education Mega Center, Guangzhou 510006, China. Tel: +86 13924061779, +86 20 39943041.A high-speed counter-current chromatography (HSCCC) method was successfully developed for the preparative separation and purification of deoxyschizandrin from Schisandrae Sphenantherae Fructus in one step. The purity of deoxyschizandrin was 98.5%, and the structure was identified by MS, UV and NMR. This method was simple, fast, convenient and appropriate to prepare pure compound as reference substances for related research on Schisandrae Sphenantherae Fructus. Keywords: Schisandrae Sphenantherae Fructus, High-speed counter-current chromatography (HSCCC), Deoxyschizandrinhttp://www.sciencedirect.com/science/article/pii/S2095177913000713
collection DOAJ
language English
format Article
sources DOAJ
author Xin-Ying Li
Mei Yang
Jie-Yun Huang
Xiao-Xue Yu
Min-Qian Zhao
Zhi-Kun Liang
Zhi-Sheng Xie
Xin-Jun Xu
spellingShingle Xin-Ying Li
Mei Yang
Jie-Yun Huang
Xiao-Xue Yu
Min-Qian Zhao
Zhi-Kun Liang
Zhi-Sheng Xie
Xin-Jun Xu
Preparative separation and purification of deoxyschizandrin from Schisandrae Sphenantherae Fructus by high-speed counter-current chromatography
Journal of Pharmaceutical Analysis
author_facet Xin-Ying Li
Mei Yang
Jie-Yun Huang
Xiao-Xue Yu
Min-Qian Zhao
Zhi-Kun Liang
Zhi-Sheng Xie
Xin-Jun Xu
author_sort Xin-Ying Li
title Preparative separation and purification of deoxyschizandrin from Schisandrae Sphenantherae Fructus by high-speed counter-current chromatography
title_short Preparative separation and purification of deoxyschizandrin from Schisandrae Sphenantherae Fructus by high-speed counter-current chromatography
title_full Preparative separation and purification of deoxyschizandrin from Schisandrae Sphenantherae Fructus by high-speed counter-current chromatography
title_fullStr Preparative separation and purification of deoxyschizandrin from Schisandrae Sphenantherae Fructus by high-speed counter-current chromatography
title_full_unstemmed Preparative separation and purification of deoxyschizandrin from Schisandrae Sphenantherae Fructus by high-speed counter-current chromatography
title_sort preparative separation and purification of deoxyschizandrin from schisandrae sphenantherae fructus by high-speed counter-current chromatography
publisher Elsevier
series Journal of Pharmaceutical Analysis
issn 2095-1779
publishDate 2013-12-01
description A high-speed counter-current chromatography (HSCCC) method was successfully developed for the preparative separation and purification of deoxyschizandrin from Schisandrae Sphenantherae Fructus in one step. The purity of deoxyschizandrin was 98.5%, and the structure was identified by MS, UV and NMR. This method was simple, fast, convenient and appropriate to prepare pure compound as reference substances for related research on Schisandrae Sphenantherae Fructus. Keywords: Schisandrae Sphenantherae Fructus, High-speed counter-current chromatography (HSCCC), Deoxyschizandrin
url http://www.sciencedirect.com/science/article/pii/S2095177913000713
work_keys_str_mv AT xinyingli preparativeseparationandpurificationofdeoxyschizandrinfromschisandraesphenantheraefructusbyhighspeedcountercurrentchromatography
AT meiyang preparativeseparationandpurificationofdeoxyschizandrinfromschisandraesphenantheraefructusbyhighspeedcountercurrentchromatography
AT jieyunhuang preparativeseparationandpurificationofdeoxyschizandrinfromschisandraesphenantheraefructusbyhighspeedcountercurrentchromatography
AT xiaoxueyu preparativeseparationandpurificationofdeoxyschizandrinfromschisandraesphenantheraefructusbyhighspeedcountercurrentchromatography
AT minqianzhao preparativeseparationandpurificationofdeoxyschizandrinfromschisandraesphenantheraefructusbyhighspeedcountercurrentchromatography
AT zhikunliang preparativeseparationandpurificationofdeoxyschizandrinfromschisandraesphenantheraefructusbyhighspeedcountercurrentchromatography
AT zhishengxie preparativeseparationandpurificationofdeoxyschizandrinfromschisandraesphenantheraefructusbyhighspeedcountercurrentchromatography
AT xinjunxu preparativeseparationandpurificationofdeoxyschizandrinfromschisandraesphenantheraefructusbyhighspeedcountercurrentchromatography
_version_ 1724168419566157824

Similar Items