FT-IR Transflection Micro-Spectroscopy Study on Normal Human Breast Cells after Exposure to a Proton Beam

Fourier transform infrared micro-spectroscopy (μ-FT-IR) is nowadays considered a valuable tool for investigating the changes occurring in human cells after exposure to ionizing radiation. Recently, considerable attention has been devoted to the use of this optical technique in the study of cells exp...

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Main Authors: Valerio Ricciardi, Marianna Portaccio, Giuseppe Perna, Maria Lasalvia, Vito Capozzi, Francesco Paolo Cammarata, Pietro Pisciotta, Giada Petringa, Ines Delfino, Lorenzo Manti, Maria Lepore
Format: Article
Language:English
Published: MDPI AG 2021-01-01
Series:Applied Sciences
Subjects:
Online Access:https://www.mdpi.com/2076-3417/11/2/540
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spelling doaj-57dc57ab849a4e49ba70d64a44a8059c2021-01-08T00:04:40ZengMDPI AGApplied Sciences2076-34172021-01-011154054010.3390/app11020540FT-IR Transflection Micro-Spectroscopy Study on Normal Human Breast Cells after Exposure to a Proton BeamValerio Ricciardi0Marianna Portaccio1Giuseppe Perna2Maria Lasalvia3Vito Capozzi4Francesco Paolo Cammarata5Pietro Pisciotta6Giada Petringa7Ines Delfino8Lorenzo Manti9Maria Lepore10Dipartimento di Medicina Sperimentale, Università della Campania “Luigi Vanvitelli”, 80138 Napoli, ItalyDipartimento di Medicina Sperimentale, Università della Campania “Luigi Vanvitelli”, 80138 Napoli, ItalyDipartimento di Medicina Clinica e Sperimentale, Università di Foggia, 71122 Foggia, ItalyDipartimento di Medicina Clinica e Sperimentale, Università di Foggia, 71122 Foggia, ItalyDipartimento di Medicina Clinica e Sperimentale, Università di Foggia, 71122 Foggia, ItalyIstituto di Bioimmagini e Fisiologia Molecolare—Consiglio Nazionale delle Ricerche, 90015 Cefalù, ItalyIstituto Nazionale di Fisica Nucleare—Laboratori Nazionali del Sud, 95123 Catania, ItalyIstituto Nazionale di Fisica Nucleare—Laboratori Nazionali del Sud, 95123 Catania, ItalyDipartimento di Scienze Ecologiche e Biologiche, Università della Tuscia, 01100 Viterbo, ItalyIstituto Nazionale di Fisica Nucleare—Sezione di Napoli, 80100 Napoli, ItalyDipartimento di Medicina Sperimentale, Università della Campania “Luigi Vanvitelli”, 80138 Napoli, ItalyFourier transform infrared micro-spectroscopy (μ-FT-IR) is nowadays considered a valuable tool for investigating the changes occurring in human cells after exposure to ionizing radiation. Recently, considerable attention has been devoted to the use of this optical technique in the study of cells exposed to proton beams, that are being increasingly adopted in cancer therapy. Different experimental configurations are used for proton irradiation and subsequent spectra acquisition. To facilitate the use of μ-FT-IR, it may be useful to investigate new experimental approaches capable of speeding up and simplifying the irradiation and measurements phases. Here, we propose the use of low-e-substrates slides for cell culture, allowing the irradiation and spectra acquisition in transflection mode in a fast and direct way. In recent years, there has been a wide debate about the validity of these supports, but many researchers agree that the artifacts due to the presence of the electromagnetic standing wave effects are negligible in many practical cases. We investigated human normal breast cells (MCF-10 cell line) fixed immediately after the irradiation with graded proton radiation doses (0, 0.5, 2, and 4 Gy). The spectra obtained in transflection geometry showed characteristics very similar to those present in the spectra acquired in transmission geometry and confirm the validity of the chosen approach. The analysis of spectra indicates the occurrence of significant changes in DNA and lipids components of cells. Modifications in protein secondary structure are also evidenced.https://www.mdpi.com/2076-3417/11/2/540fourier transform infrared micro-spectroscopytransflection geometryproton therapyradiation dose effectshuman breast cells
collection DOAJ
language English
format Article
sources DOAJ
author Valerio Ricciardi
Marianna Portaccio
Giuseppe Perna
Maria Lasalvia
Vito Capozzi
Francesco Paolo Cammarata
Pietro Pisciotta
Giada Petringa
Ines Delfino
Lorenzo Manti
Maria Lepore
spellingShingle Valerio Ricciardi
Marianna Portaccio
Giuseppe Perna
Maria Lasalvia
Vito Capozzi
Francesco Paolo Cammarata
Pietro Pisciotta
Giada Petringa
Ines Delfino
Lorenzo Manti
Maria Lepore
FT-IR Transflection Micro-Spectroscopy Study on Normal Human Breast Cells after Exposure to a Proton Beam
Applied Sciences
fourier transform infrared micro-spectroscopy
transflection geometry
proton therapy
radiation dose effects
human breast cells
author_facet Valerio Ricciardi
Marianna Portaccio
Giuseppe Perna
Maria Lasalvia
Vito Capozzi
Francesco Paolo Cammarata
Pietro Pisciotta
Giada Petringa
Ines Delfino
Lorenzo Manti
Maria Lepore
author_sort Valerio Ricciardi
title FT-IR Transflection Micro-Spectroscopy Study on Normal Human Breast Cells after Exposure to a Proton Beam
title_short FT-IR Transflection Micro-Spectroscopy Study on Normal Human Breast Cells after Exposure to a Proton Beam
title_full FT-IR Transflection Micro-Spectroscopy Study on Normal Human Breast Cells after Exposure to a Proton Beam
title_fullStr FT-IR Transflection Micro-Spectroscopy Study on Normal Human Breast Cells after Exposure to a Proton Beam
title_full_unstemmed FT-IR Transflection Micro-Spectroscopy Study on Normal Human Breast Cells after Exposure to a Proton Beam
title_sort ft-ir transflection micro-spectroscopy study on normal human breast cells after exposure to a proton beam
publisher MDPI AG
series Applied Sciences
issn 2076-3417
publishDate 2021-01-01
description Fourier transform infrared micro-spectroscopy (μ-FT-IR) is nowadays considered a valuable tool for investigating the changes occurring in human cells after exposure to ionizing radiation. Recently, considerable attention has been devoted to the use of this optical technique in the study of cells exposed to proton beams, that are being increasingly adopted in cancer therapy. Different experimental configurations are used for proton irradiation and subsequent spectra acquisition. To facilitate the use of μ-FT-IR, it may be useful to investigate new experimental approaches capable of speeding up and simplifying the irradiation and measurements phases. Here, we propose the use of low-e-substrates slides for cell culture, allowing the irradiation and spectra acquisition in transflection mode in a fast and direct way. In recent years, there has been a wide debate about the validity of these supports, but many researchers agree that the artifacts due to the presence of the electromagnetic standing wave effects are negligible in many practical cases. We investigated human normal breast cells (MCF-10 cell line) fixed immediately after the irradiation with graded proton radiation doses (0, 0.5, 2, and 4 Gy). The spectra obtained in transflection geometry showed characteristics very similar to those present in the spectra acquired in transmission geometry and confirm the validity of the chosen approach. The analysis of spectra indicates the occurrence of significant changes in DNA and lipids components of cells. Modifications in protein secondary structure are also evidenced.
topic fourier transform infrared micro-spectroscopy
transflection geometry
proton therapy
radiation dose effects
human breast cells
url https://www.mdpi.com/2076-3417/11/2/540
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