Gastrodin protects dopaminergic neurons via insulin-like pathway in a Parkinson’s disease model

Abstract Background Recently, the use of traditional Chinese medicine (TCM) has become more generally accepted, including by the Food and Drug Administration. To expand the use of TCM worldwide, it is important to study the molecular mechanisms by which TCM and its active ingredients produce effects...

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Main Authors: Jinyuan Yan, Zhongshan Yang, Ninghui Zhao, Zhiwei Li, Xia Cao
Format: Article
Language:English
Published: BMC 2019-06-01
Series:BMC Neuroscience
Subjects:
Online Access:http://link.springer.com/article/10.1186/s12868-019-0512-x
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spelling doaj-57d232ff1fd745f0ac4c7a640d209b892020-11-25T03:08:07ZengBMCBMC Neuroscience1471-22022019-06-0120111110.1186/s12868-019-0512-xGastrodin protects dopaminergic neurons via insulin-like pathway in a Parkinson’s disease modelJinyuan Yan0Zhongshan Yang1Ninghui Zhao2Zhiwei Li3Xia Cao4The Second Affiliated Hospital of Kunming Medical UniversityFaculty of Basic Medicine, Yunnan University of Chinese MedicineThe Second Affiliated Hospital of Kunming Medical UniversityThe Second Affiliated Hospital of Kunming Medical UniversityThe Second Affiliated Hospital of Kunming Medical UniversityAbstract Background Recently, the use of traditional Chinese medicine (TCM) has become more generally accepted, including by the Food and Drug Administration. To expand the use of TCM worldwide, it is important to study the molecular mechanisms by which TCM and its active ingredients produce effects. Gastrodin is an active ingredient from Gastrodia elata Blume. It is reported that gastrodin has neuroprotective function in Parkinson’s disease. But its mechanisms of neuroprotection remain not clear in PD. Here, we build two C. elegans PD model using 6-OHDA and transgenic animal to observe the changes of PD worms treated with or without gastrodin to confirm the function of gastrodin, then utilize mutant worms to investigate DAF-2/DAF-16 signaling pathway, and finally verify the mechanism of gastrodin in PD. Results Gastrodin attenuates the accumulation of α-synuclein and the injury of dopaminergic neurons, improves chemotaxis behavior in Parkinson’s disease models, then recovers chemotaxis behavior by insulin-like pathway. DAF-2/DAF-16 is required for neuroprotective effect of dopamine neuron in PD. Conclusions Our study demonstrated that gastrodin rescued dopaminergic neurons and reduced accumulation of α-synuclein protein, and the activity of gastrodin against Parkinson’s disease depended on the insulin-like DAF-2/DAF-16 signaling pathway. Our findings revealed that this insulin-like pathway mediates neuroprotection of gastrodin in a Parkinson’s disease model.http://link.springer.com/article/10.1186/s12868-019-0512-xGastrodinDopamine neuronsα-SynucleinDAF-2/DAF-16 pathwayParkinson’s disease
collection DOAJ
language English
format Article
sources DOAJ
author Jinyuan Yan
Zhongshan Yang
Ninghui Zhao
Zhiwei Li
Xia Cao
spellingShingle Jinyuan Yan
Zhongshan Yang
Ninghui Zhao
Zhiwei Li
Xia Cao
Gastrodin protects dopaminergic neurons via insulin-like pathway in a Parkinson’s disease model
BMC Neuroscience
Gastrodin
Dopamine neurons
α-Synuclein
DAF-2/DAF-16 pathway
Parkinson’s disease
author_facet Jinyuan Yan
Zhongshan Yang
Ninghui Zhao
Zhiwei Li
Xia Cao
author_sort Jinyuan Yan
title Gastrodin protects dopaminergic neurons via insulin-like pathway in a Parkinson’s disease model
title_short Gastrodin protects dopaminergic neurons via insulin-like pathway in a Parkinson’s disease model
title_full Gastrodin protects dopaminergic neurons via insulin-like pathway in a Parkinson’s disease model
title_fullStr Gastrodin protects dopaminergic neurons via insulin-like pathway in a Parkinson’s disease model
title_full_unstemmed Gastrodin protects dopaminergic neurons via insulin-like pathway in a Parkinson’s disease model
title_sort gastrodin protects dopaminergic neurons via insulin-like pathway in a parkinson’s disease model
publisher BMC
series BMC Neuroscience
issn 1471-2202
publishDate 2019-06-01
description Abstract Background Recently, the use of traditional Chinese medicine (TCM) has become more generally accepted, including by the Food and Drug Administration. To expand the use of TCM worldwide, it is important to study the molecular mechanisms by which TCM and its active ingredients produce effects. Gastrodin is an active ingredient from Gastrodia elata Blume. It is reported that gastrodin has neuroprotective function in Parkinson’s disease. But its mechanisms of neuroprotection remain not clear in PD. Here, we build two C. elegans PD model using 6-OHDA and transgenic animal to observe the changes of PD worms treated with or without gastrodin to confirm the function of gastrodin, then utilize mutant worms to investigate DAF-2/DAF-16 signaling pathway, and finally verify the mechanism of gastrodin in PD. Results Gastrodin attenuates the accumulation of α-synuclein and the injury of dopaminergic neurons, improves chemotaxis behavior in Parkinson’s disease models, then recovers chemotaxis behavior by insulin-like pathway. DAF-2/DAF-16 is required for neuroprotective effect of dopamine neuron in PD. Conclusions Our study demonstrated that gastrodin rescued dopaminergic neurons and reduced accumulation of α-synuclein protein, and the activity of gastrodin against Parkinson’s disease depended on the insulin-like DAF-2/DAF-16 signaling pathway. Our findings revealed that this insulin-like pathway mediates neuroprotection of gastrodin in a Parkinson’s disease model.
topic Gastrodin
Dopamine neurons
α-Synuclein
DAF-2/DAF-16 pathway
Parkinson’s disease
url http://link.springer.com/article/10.1186/s12868-019-0512-x
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AT zhiweili gastrodinprotectsdopaminergicneuronsviainsulinlikepathwayinaparkinsonsdiseasemodel
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