Cytological features of live limbal tissue donor eyes for autograft or allograft limbal stem cell transplantation
PURPOSE: To evaluate by impression cytology (IC) the corneal surface of live limbal tissue donor eyes for autograft or allograft limbal stem cell transplantation (LSCT). METHODS: Twenty limbal donors were enrolled (17 for autograft LSCT and 3 for allograft). Impression cytology was performed before...
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Conselho Brasileiro de Oftalmologia
2011-08-01
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doaj-57cdadda17ea4585ae3ca12412fd2fa72020-11-24T23:05:46ZengConselho Brasileiro de OftalmologiaArquivos Brasileiros de Oftalmologia1678-29252011-08-0174424825010.1590/S0004-27492011000400003S0004-27492011000400003Cytological features of live limbal tissue donor eyes for autograft or allograft limbal stem cell transplantationJeison de Nadai Barros0Myrna Serapião dos Santos1Telma Regina Maria Pereira Barreiro2Rubens Belfort Jr3José Álvaro Pereira Gomes4Universidade Federal de São PauloUniversidade Federal de São PauloUniversidade Federal de São PauloUniversidade Federal de São PauloUniversidade Federal de São PauloPURPOSE: To evaluate by impression cytology (IC) the corneal surface of live limbal tissue donor eyes for autograft or allograft limbal stem cell transplantation (LSCT). METHODS: Twenty limbal donors were enrolled (17 for autograft LSCT and 3 for allograft). Impression cytology was performed before transplantation of superior and inferior limbal grafts and after the third postoperative month. RESULTS: Impression cytology analysis showed sheets of corneal epithelial cells and goblet cell absence beyond the edge of the keratectomy sites in all patients, suggesting that conjunctival invasion towards the center did not occur in any eye. Partial conjunctivalization within 2 to 3 clock hours, confirmed by the presence of goblet cells, was limited to the keratectomy site in 10% of the cases. CONCLUSION: A clear central corneal surface was demonstrated in all eyes following surgery leading to the conclusion that limbal donation was a safe procedure in this group of patients. A small percentage of eyes can have donor sites re-epithelized with conjunctival cells at the periphery of the cornea.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492011000400003&lng=en&tlng=enCélulas-troncoTécnicas citológicasLimbo da córneaCélulas caliciformesCélulas epiteliaisDoadores vivosTransplante autólogo |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jeison de Nadai Barros Myrna Serapião dos Santos Telma Regina Maria Pereira Barreiro Rubens Belfort Jr José Álvaro Pereira Gomes |
spellingShingle |
Jeison de Nadai Barros Myrna Serapião dos Santos Telma Regina Maria Pereira Barreiro Rubens Belfort Jr José Álvaro Pereira Gomes Cytological features of live limbal tissue donor eyes for autograft or allograft limbal stem cell transplantation Arquivos Brasileiros de Oftalmologia Células-tronco Técnicas citológicas Limbo da córnea Células caliciformes Células epiteliais Doadores vivos Transplante autólogo |
author_facet |
Jeison de Nadai Barros Myrna Serapião dos Santos Telma Regina Maria Pereira Barreiro Rubens Belfort Jr José Álvaro Pereira Gomes |
author_sort |
Jeison de Nadai Barros |
title |
Cytological features of live limbal tissue donor eyes for autograft or allograft limbal stem cell transplantation |
title_short |
Cytological features of live limbal tissue donor eyes for autograft or allograft limbal stem cell transplantation |
title_full |
Cytological features of live limbal tissue donor eyes for autograft or allograft limbal stem cell transplantation |
title_fullStr |
Cytological features of live limbal tissue donor eyes for autograft or allograft limbal stem cell transplantation |
title_full_unstemmed |
Cytological features of live limbal tissue donor eyes for autograft or allograft limbal stem cell transplantation |
title_sort |
cytological features of live limbal tissue donor eyes for autograft or allograft limbal stem cell transplantation |
publisher |
Conselho Brasileiro de Oftalmologia |
series |
Arquivos Brasileiros de Oftalmologia |
issn |
1678-2925 |
publishDate |
2011-08-01 |
description |
PURPOSE: To evaluate by impression cytology (IC) the corneal surface of live limbal tissue donor eyes for autograft or allograft limbal stem cell transplantation (LSCT). METHODS: Twenty limbal donors were enrolled (17 for autograft LSCT and 3 for allograft). Impression cytology was performed before transplantation of superior and inferior limbal grafts and after the third postoperative month. RESULTS: Impression cytology analysis showed sheets of corneal epithelial cells and goblet cell absence beyond the edge of the keratectomy sites in all patients, suggesting that conjunctival invasion towards the center did not occur in any eye. Partial conjunctivalization within 2 to 3 clock hours, confirmed by the presence of goblet cells, was limited to the keratectomy site in 10% of the cases. CONCLUSION: A clear central corneal surface was demonstrated in all eyes following surgery leading to the conclusion that limbal donation was a safe procedure in this group of patients. A small percentage of eyes can have donor sites re-epithelized with conjunctival cells at the periphery of the cornea. |
topic |
Células-tronco Técnicas citológicas Limbo da córnea Células caliciformes Células epiteliais Doadores vivos Transplante autólogo |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492011000400003&lng=en&tlng=en |
work_keys_str_mv |
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