Cytological features of live limbal tissue donor eyes for autograft or allograft limbal stem cell transplantation

PURPOSE: To evaluate by impression cytology (IC) the corneal surface of live limbal tissue donor eyes for autograft or allograft limbal stem cell transplantation (LSCT). METHODS: Twenty limbal donors were enrolled (17 for autograft LSCT and 3 for allograft). Impression cytology was performed before...

Full description

Bibliographic Details
Main Authors: Jeison de Nadai Barros, Myrna Serapião dos Santos, Telma Regina Maria Pereira Barreiro, Rubens Belfort Jr, José Álvaro Pereira Gomes
Format: Article
Language:English
Published: Conselho Brasileiro de Oftalmologia 2011-08-01
Series:Arquivos Brasileiros de Oftalmologia
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492011000400003&lng=en&tlng=en
id doaj-57cdadda17ea4585ae3ca12412fd2fa7
record_format Article
spelling doaj-57cdadda17ea4585ae3ca12412fd2fa72020-11-24T23:05:46ZengConselho Brasileiro de OftalmologiaArquivos Brasileiros de Oftalmologia1678-29252011-08-0174424825010.1590/S0004-27492011000400003S0004-27492011000400003Cytological features of live limbal tissue donor eyes for autograft or allograft limbal stem cell transplantationJeison de Nadai Barros0Myrna Serapião dos Santos1Telma Regina Maria Pereira Barreiro2Rubens Belfort Jr3José Álvaro Pereira Gomes4Universidade Federal de São PauloUniversidade Federal de São PauloUniversidade Federal de São PauloUniversidade Federal de São PauloUniversidade Federal de São PauloPURPOSE: To evaluate by impression cytology (IC) the corneal surface of live limbal tissue donor eyes for autograft or allograft limbal stem cell transplantation (LSCT). METHODS: Twenty limbal donors were enrolled (17 for autograft LSCT and 3 for allograft). Impression cytology was performed before transplantation of superior and inferior limbal grafts and after the third postoperative month. RESULTS: Impression cytology analysis showed sheets of corneal epithelial cells and goblet cell absence beyond the edge of the keratectomy sites in all patients, suggesting that conjunctival invasion towards the center did not occur in any eye. Partial conjunctivalization within 2 to 3 clock hours, confirmed by the presence of goblet cells, was limited to the keratectomy site in 10% of the cases. CONCLUSION: A clear central corneal surface was demonstrated in all eyes following surgery leading to the conclusion that limbal donation was a safe procedure in this group of patients. A small percentage of eyes can have donor sites re-epithelized with conjunctival cells at the periphery of the cornea.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492011000400003&lng=en&tlng=enCélulas-troncoTécnicas citológicasLimbo da córneaCélulas caliciformesCélulas epiteliaisDoadores vivosTransplante autólogo
collection DOAJ
language English
format Article
sources DOAJ
author Jeison de Nadai Barros
Myrna Serapião dos Santos
Telma Regina Maria Pereira Barreiro
Rubens Belfort Jr
José Álvaro Pereira Gomes
spellingShingle Jeison de Nadai Barros
Myrna Serapião dos Santos
Telma Regina Maria Pereira Barreiro
Rubens Belfort Jr
José Álvaro Pereira Gomes
Cytological features of live limbal tissue donor eyes for autograft or allograft limbal stem cell transplantation
Arquivos Brasileiros de Oftalmologia
Células-tronco
Técnicas citológicas
Limbo da córnea
Células caliciformes
Células epiteliais
Doadores vivos
Transplante autólogo
author_facet Jeison de Nadai Barros
Myrna Serapião dos Santos
Telma Regina Maria Pereira Barreiro
Rubens Belfort Jr
José Álvaro Pereira Gomes
author_sort Jeison de Nadai Barros
title Cytological features of live limbal tissue donor eyes for autograft or allograft limbal stem cell transplantation
title_short Cytological features of live limbal tissue donor eyes for autograft or allograft limbal stem cell transplantation
title_full Cytological features of live limbal tissue donor eyes for autograft or allograft limbal stem cell transplantation
title_fullStr Cytological features of live limbal tissue donor eyes for autograft or allograft limbal stem cell transplantation
title_full_unstemmed Cytological features of live limbal tissue donor eyes for autograft or allograft limbal stem cell transplantation
title_sort cytological features of live limbal tissue donor eyes for autograft or allograft limbal stem cell transplantation
publisher Conselho Brasileiro de Oftalmologia
series Arquivos Brasileiros de Oftalmologia
issn 1678-2925
publishDate 2011-08-01
description PURPOSE: To evaluate by impression cytology (IC) the corneal surface of live limbal tissue donor eyes for autograft or allograft limbal stem cell transplantation (LSCT). METHODS: Twenty limbal donors were enrolled (17 for autograft LSCT and 3 for allograft). Impression cytology was performed before transplantation of superior and inferior limbal grafts and after the third postoperative month. RESULTS: Impression cytology analysis showed sheets of corneal epithelial cells and goblet cell absence beyond the edge of the keratectomy sites in all patients, suggesting that conjunctival invasion towards the center did not occur in any eye. Partial conjunctivalization within 2 to 3 clock hours, confirmed by the presence of goblet cells, was limited to the keratectomy site in 10% of the cases. CONCLUSION: A clear central corneal surface was demonstrated in all eyes following surgery leading to the conclusion that limbal donation was a safe procedure in this group of patients. A small percentage of eyes can have donor sites re-epithelized with conjunctival cells at the periphery of the cornea.
topic Células-tronco
Técnicas citológicas
Limbo da córnea
Células caliciformes
Células epiteliais
Doadores vivos
Transplante autólogo
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492011000400003&lng=en&tlng=en
work_keys_str_mv AT jeisondenadaibarros cytologicalfeaturesoflivelimbaltissuedonoreyesforautograftorallograftlimbalstemcelltransplantation
AT myrnaserapiaodossantos cytologicalfeaturesoflivelimbaltissuedonoreyesforautograftorallograftlimbalstemcelltransplantation
AT telmareginamariapereirabarreiro cytologicalfeaturesoflivelimbaltissuedonoreyesforautograftorallograftlimbalstemcelltransplantation
AT rubensbelfortjr cytologicalfeaturesoflivelimbaltissuedonoreyesforautograftorallograftlimbalstemcelltransplantation
AT josealvaropereiragomes cytologicalfeaturesoflivelimbaltissuedonoreyesforautograftorallograftlimbalstemcelltransplantation
_version_ 1725625779489865728