Investigating the Efficiency of Simple Aqueous Extract of Nigella Sativa Activity Against Serratia Marcescens Bactria

• Main Authors: Samir Laybi Shkhaier, Muthana Anad Majid, Wathiq Mohammad Alawi, Jamal ِAbed Al-Fatah, Hayder Hameed Abd
• Format: Article
• Language: Arabic
• Published: Al-Mustansiriyah University 2020-08-01
• Series: Mustansiriyah Journal of Science
• Subjects: nigella sativa, serratia marcescens, water nigella extract activity, treatment of serratia marcescens with nigella sativa.
• Online Access: http://mjs.uomustansiriyah.edu.iq/ojs1/index.php/MJS/article/view/859

Nigella sativa seeds is considered as a very effective treatment for a variety of human diseases in traditional medicine worldwide with low human toxicity. Serratia marcescens pathogenic species, a Gram-negative, opportunistic bacteria, are widespread and distributed in the human environment with considered resistance to different types of antibiotics. Extraction was performed on 12.5 gm. /150 ml of Nigella seeds using rotary evaporator. Different time periods for extraction were applied (1-4 hours) with different temperature ranges of 50 °C to 60 °C performed separately for each time period. The 3 and 4 hours extraction period at 60 °C for seeds was effective against Serratia marcescens with inhibition zone of 11.18 and 12.27 mm respectively. Nigella seeds could be a promising inhibitor with enhanced activity against Serratia marcescens. Water extraction was efficient and effective at 60 °C, with extraction period of 2-3 hours for seeds. No activity recorded below this temperature.