Isolation and Molecular Characteristics of a CIAV Isolate From Pigeons, China

• Main Authors: Hongxia Shao, Jinzhi Li, Huisha Yuan, Lifei Ji, Jun Zhang, Wenjie Jin, Kun Qian, Jianqiang Ye, Aijian Qin
• Format: Article
• Language: English
• Published: Frontiers Media S.A. 2021-08-01
• Series: Frontiers in Veterinary Science
• Subjects: CIAV; pigeon; isolation; identification; molecular characteristics
• Online Access: https://www.frontiersin.org/articles/10.3389/fvets.2021.669154/full
• ISSN: 2297-1769

Chicken infectious anemia virus (CIAV) mainly infects chickens and causes immunosuppression. In this study, a CIAV isolate, designated as Pigeon-CIAV-1906, was efficiently isolated from two sick pigeons by inoculating the samples into MSB1 cells. The genome of Pigeon-CIAV-1906 was amplified by PCR and analyzed. The genome size of Pigeon-CIAV-1906 was 2,298 bp with the highest homology (99.5%) to Jilin strain (JL14023) and the lowest homology (91.5%) to Brazil strain (KY024579), which phylogenetically clustered into Group A. Notably, several amino acids such as 139K and 394Q related with high virulence were found in the VP1 of Pigeon-CIAV-1906. The isolation of Pigeon-CIAV-1906 and its molecular characteristics provide evidence for the cross-transmission of CIAV from chicken to pigeon and give novel insights into the molecular epidemiology of CIAV.