A preliminary analysis of the aspartateaminotransferase isozymes in Gleditsia triacanthos and Robinia pseudacacia germinating seeds

• Main Authors: Eugen Ungureanu, Vlad Artenie, Alice Madalina Ungureanu
• Format: Article
• Language: English
• Published: "Alexandru Ioan Cuza" University of Iași 2009-12-01
• Series: Analele Ştiinţifice Ale Universităţii Alexandru Ioan Cuza din Iași,Sectiunea II A : Genetica si Biologie Moleculara
• Online Access: http://www.gbm.bio.uaic.ro/index.php/gbm/article/view/547

The extracts made with sodium/potassium phosphate buffer from the probes collected during germination of the seeds from Gleditsia triacanthos and Robinia pseudacacia was subjected to polyacrylamide gel electrophoresis under native conditions. Then the solution for specific coloring of the bands in which are found the izoenzymes of aspartate-aminotransferase was poured over the gel. The coloring reaction was stopped by rinsing the gel with distilled water when the intensity of the coloration was considered optimal. The results were interesting showing the existence of more than one spot corresponding to aspartate-aminotransferase in almost all analyzed probes. At least one of the spots from each of the probes presents the same Rf as the standard we have used (SigmaAldrich Glutamic- Oxalacetic Transaminase from porcine heart, Type II-A, lyophilized powder, 100-400 units/mg protein). The main purpose of the paper was to find if there are any different isozymes of the aspartate-aminotransferase in the extracts made with sodium/potassium phosphate buffer from the probes collected during germination of the seeds from Gleditsia triacanthos and Robinia pseudacac