A preliminary analysis of the aspartateaminotransferase isozymes in Gleditsia triacanthos and Robinia pseudacacia germinating seeds
The extracts made with sodium/potassium phosphate buffer from the probes collected during germination of the seeds from Gleditsia triacanthos and Robinia pseudacacia was subjected to polyacrylamide gel electrophoresis under native conditions. Then the solution for specific coloring of the bands in w...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
"Alexandru Ioan Cuza" University of Iași
2009-12-01
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| Series: | Analele Ştiinţifice Ale Universităţii Alexandru Ioan Cuza din Iași,Sectiunea II A : Genetica si Biologie Moleculara |
| Online Access: | http://www.gbm.bio.uaic.ro/index.php/gbm/article/view/547 |
| Summary: | The extracts made with sodium/potassium phosphate buffer from the probes collected during
germination of the seeds from Gleditsia triacanthos and Robinia pseudacacia was subjected to polyacrylamide gel
electrophoresis under native conditions. Then the solution for specific coloring of the bands in which are found the
izoenzymes of aspartate-aminotransferase was poured over the gel. The coloring reaction was stopped by rinsing the gel
with distilled water when the intensity of the coloration was considered optimal. The results were interesting showing the
existence of more than one spot corresponding to aspartate-aminotransferase in almost all analyzed probes. At least one
of the spots from each of the probes presents the same Rf as the standard we have used (SigmaAldrich Glutamic-
Oxalacetic Transaminase from porcine heart, Type II-A, lyophilized powder, 100-400 units/mg protein).
The main purpose of the paper was to find if there are any different isozymes of the aspartate-aminotransferase
in the extracts made with sodium/potassium phosphate buffer from the probes collected during germination of the seeds
from Gleditsia triacanthos and Robinia pseudacac |
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| ISSN: | 1582-3571 2248-3276 |