| Summary: | In this study, it was aimed to survey the vineyards for the presence of the Grapevine rupestris stem pitting-associated virus (GRSPaV) in the Southeastern Anatolia (Gaziantep, Adıyaman and Kilis) and Eastern Mediterranean Region (Kahramanmaraş, Hatay, Adana and Mersin) with a newly designed primer pair. A total of 381 samples from 96 vineyards were collected and analysed by RT-PCR technique. Of the 381 samples collected, 40 were found to be infected with GRSPaV. The prevalence rates of GRSPaV were respectively calculated as follows: Kahramanmaras (4.46%), Kilis (2.36%), Adıyaman (1.58%), Gaziantep (1.31%), Adana (0.53%), Mersin (0.26%) and Hatay (0%). As a result, it has been determined that the virus has a significant prevalence in Southeastern Anatolia and Eastern Mediterranean Region.
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