Single-site glycine-specific labeling of proteins

Single-site glycine-specific labeling of proteins

Single-site labelling of proteins is desirable, e.g., for analytical purposes. Here, the authors developed a method in which they use an aldol-type reaction to modify proteins at N-terminal glycine residues in an efficient and selective manner, which is also applicable to cell lysates.

Bibliographic Details
Main Authors: Landa Purushottam, Srinivasa Rao Adusumalli, Usha Singh, V. B. Unnikrishnan, Dattatraya Gautam Rawale, Mansi Gujrati, Ram Kumar Mishra, Vishal Rai
Format: Article
Language:English
Published: Nature Publishing Group 2019-06-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-019-10503-7
Description
Summary:Single-site labelling of proteins is desirable, e.g., for analytical purposes. Here, the authors developed a method in which they use an aldol-type reaction to modify proteins at N-terminal glycine residues in an efficient and selective manner, which is also applicable to cell lysates.
ISSN:2041-1723

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