EF-1α silencing by feeding RNAi suppresses resting cyst formation in Colpoda cucullus Nag-1 strain

EF-1α silencing by feeding RNAi suppresses resting cyst formation in Colpoda cucullus Nag-1 strain

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It is reported that the expression level of EF-1α in Colpoda cucullus Nag-1 is markedly enhanced within several hours after the onset of encystment induction. In this study, the Colpoda strain (stock EQ-1) known to promptly encyst also showed early EF-1α expression while the strain (stock ES-1) k...

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Main Authors: Y Sogame, M Hori, T Matsuoka
Format: Article
Language:English
Published: University of Modena and Reggio Emilia 2016-03-01
Series:Invertebrate Survival Journal
Subjects:
EF-1α; Colpoda; encystment; feeding RNAi
Online Access:http://www.isj.unimo.it/articoli/ISJ409.pdf
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spelling doaj-574344e951924d3e9c1990f323b0d7a82020-11-25T03:27:01ZengUniversity of Modena and Reggio EmiliaInvertebrate Survival Journal1824-307X2016-03-01138993EF-1α silencing by feeding RNAi suppresses resting cyst formation in Colpoda cucullus Nag-1 strainY Sogame0M Hori1T Matsuoka2Department of Biological Science, Faculty of Science, Kochi University, Kochi 780-8520, Japan Division of Environmental Science and Engineering, Graduate School of Science and Engineering, Yamaguchi University,Yamaguchi 753-8512, JapanDepartment of Biological Science, Faculty of Science, Kochi University, Kochi 780-8520, Japan It is reported that the expression level of EF-1α in Colpoda cucullus Nag-1 is markedly enhanced within several hours after the onset of encystment induction. In this study, the Colpoda strain (stock EQ-1) known to promptly encyst also showed early EF-1α expression while the strain (stock ES-1) known to show prolonged encystment also showed delayed EF-1α expression. In cells in which EF-1α is silenced by feeding RNAi, the cyst formation was prolonged, but normal cyst walls were formed. These results suggest that Colpoda EF-1α is involved in the early morphogenetic events of the resting cyst formation by accelerating protein translation or cytosleletal dynamics such as microtubule disintegration.http://www.isj.unimo.it/articoli/ISJ409.pdfEF-1α; Colpoda; encystment; feeding RNAi
collection DOAJ
language English
format Article
sources DOAJ
author Y Sogame
M Hori
T Matsuoka
spellingShingle Y Sogame
M Hori
T Matsuoka
EF-1α silencing by feeding RNAi suppresses resting cyst formation in Colpoda cucullus Nag-1 strain
Invertebrate Survival Journal
EF-1α; Colpoda; encystment; feeding RNAi
author_facet Y Sogame
M Hori
T Matsuoka
author_sort Y Sogame
title EF-1α silencing by feeding RNAi suppresses resting cyst formation in Colpoda cucullus Nag-1 strain
title_short EF-1α silencing by feeding RNAi suppresses resting cyst formation in Colpoda cucullus Nag-1 strain
title_full EF-1α silencing by feeding RNAi suppresses resting cyst formation in Colpoda cucullus Nag-1 strain
title_fullStr EF-1α silencing by feeding RNAi suppresses resting cyst formation in Colpoda cucullus Nag-1 strain
title_full_unstemmed EF-1α silencing by feeding RNAi suppresses resting cyst formation in Colpoda cucullus Nag-1 strain
title_sort ef-1α silencing by feeding rnai suppresses resting cyst formation in colpoda cucullus nag-1 strain
publisher University of Modena and Reggio Emilia
series Invertebrate Survival Journal
issn 1824-307X
publishDate 2016-03-01
description It is reported that the expression level of EF-1α in Colpoda cucullus Nag-1 is markedly enhanced within several hours after the onset of encystment induction. In this study, the Colpoda strain (stock EQ-1) known to promptly encyst also showed early EF-1α expression while the strain (stock ES-1) known to show prolonged encystment also showed delayed EF-1α expression. In cells in which EF-1α is silenced by feeding RNAi, the cyst formation was prolonged, but normal cyst walls were formed. These results suggest that Colpoda EF-1α is involved in the early morphogenetic events of the resting cyst formation by accelerating protein translation or cytosleletal dynamics such as microtubule disintegration.
topic EF-1α; Colpoda; encystment; feeding RNAi
url http://www.isj.unimo.it/articoli/ISJ409.pdf
work_keys_str_mv AT ysogame ef1asilencingbyfeedingrnaisuppressesrestingcystformationincolpodacucullusnag1strain
AT mhori ef1asilencingbyfeedingrnaisuppressesrestingcystformationincolpodacucullusnag1strain
AT tmatsuoka ef1asilencingbyfeedingrnaisuppressesrestingcystformationincolpodacucullusnag1strain
_version_ 1724589969841848320

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