Pb2+, Cu2+, Zn2+, Mg2+ and Mn2+ reduce the affinities of flavone, genistein and kaempferol for human serum albumin in vitro

• Main Authors: Yang Fan, Wang Jie, Liu Chunxi, Xiaochen Xu, Wenjun Li, Xia Zhihua, Xiao Jianbo
• Format: Article
• Language: English
• Published: University of Belgrade, University of Novi Sad 2011-01-01
• Series: Archives of Biological Sciences
• Subjects: flavone; genistein; kaempferol; human serum albumin; Pb2+; Cu2+; Zn2+; Mg2+; Mn2+
• Online Access: http://www.doiserbia.nb.rs/img/doi/0354-4664/2011/0354-46641103623Y.pdf

Flavone (Fl), genistein (Gen) and kaempferol (Kol) were studied for their affinities towards human serum albumin (HSA) in the presence and absence of Pb2+,Cu2+,Zn2+,Mg2+ and Mn2+. The fluorescence intensities of HSA decreased with increasing concentration of the three flavonoids. Kaempferol resulted in a blue-shift of the λem of HSA from 336 to 330 nm; flavone showed an obvious red-shift of the λem of HSA from 336 to 342 nm; genistein did not cause an obvious blue-shift or red-shift of the λem of HSA. However, the extents of λem-shifts induced by the flavonoids in the presence of metal ions were much bigger than that in the absence of mental ions. Pb2+,Cu2+,Zn2+,Mg2+ and Mn2+ reduced the quenching constants of the flavonoids for HSA by 14.6% to 60.7% , 28% to 67.9%,3.5% to 59.4%, 23.2% to 63.7% and 14% to 65%, respectively. The affinities of flavone, genistein and kaempferol for HSA decreased about 10.84%, 10.05%and 3.56% in the presence of Pb2+, respectively. Cu2+ decreased the affinities of flavone, genistein and kaempferol for HSA about 14.04%, 5.14%and 8.89%, respectively. Zn2+ decreased the affinities of flavone, genistein and kaempferol for HSA about 3.79%, 0.55% and 3.58%, respectively. Mg2+ decreased the affinities of flavone, genistein and kaempferol for HSA about 16.94%, 2.94%and 7.04%, respectively. Mn2+ decreased the affinities of flavone, genistein and kaempferol for HSA about 14.24%, 3.66% and 4.78%, respectively.